Disinfectants & Stabilizers

Keep your lab free of microbial contaminations

A CO2 incubator is a basic piece of equipment in any laboratory involved in cultivation of in vitro cells. The culture bottles must be kept slightly opened in order to allow the penetration of CO2, and enable the formation of a bicarbonate - CO2 buffer system, which is the most common buffer system for tissue culture. Leakage of medium from the culture bottles, high humidity, suitable temperature, the water tray and the air flow, all form a fertile breeding ground for the development of contaminants in the incubator, which are liable to contaminate the tissue culture in all the semi-opened culture bottles.
The usual way of contamination is a media spill from a contaminated cell culture during loading and unloading of the cabinet. These spills can contaminate the trays and the water reservoir. Mycoplasmas are known to survive a distinct time on smooth surfaces even after the spilled culture medium has dried out. More serious is a contamination of the water tank needed to control the moisture level. Mycoplasmas can survive and, as some experts assume, also grow in pure water.
As soon as contamination appears in the incubator, it is very difficult to eliminate it, and severe damage can be caused. Therefore, it is essential to systematically clean and disinfect the incubator. It is recommended to disinfect every 14 days, or once a month at the very least.
Also water baths are common sources of contamination in a cell culture lab which occurs when e.g. frozen cells are thawed or media bottles are warmed up.
PromoKine's Aquaguard-1 and Aquaguard-2 solutions for maintenance of water quality in ordinary water baths and CO2 incubators as well as our Mycoplasma-ExS Spray, PromoCidal Spray and Spore-EX Spray & Wipes for disinfection of laboratory surfaces (work benches, incubators etc.) reliably help you to prevent unwanted contamination in your cell culture lab.