Products

Liver Function Marker Assays

Liver function tests are measuring various liver enzymes and other nonenzymatic proteins and metabolites of liver metabolism. They provide useful information about liver dysfunction/disorders and damages, and are often used in clinical practice as well as in development of therapeutics and monitoring of medications. Those hepatic function markers include albumin, bilirubin, aspartate aminotransferase (AST/SGOT), gamma glutamyl transferase (GGT), alanine aminotransferase (ALT/SGPT), alkaline phosphatase (ALP), and lactate dehydrogenase.


Gamma-glutamyltransferase (GGT) plays a key role in the synthesis of the antioxidant glutathione and thus in drug and xenobiotic detoxification. As part of glutathione recycling, it catalyzes the transfer of gamma-glutamyl functional groups from molecules such as glutathione to an acceptor that may be an amino acid, a peptide or water (forming glutamate). GGT is found in cell membranes of many tissues mainly in the liver, kidney, and pancreas. It is a diagnostic marker for various disorders that has traditionally been measured to detect liver health. High GGT levels have also been linked to increased risk of cancer, diabetes or cardiovascular mortality.

PromoKine's Gamma Glutamyl Transferase (GGT) Activity Assay Kits provide convenient tools for sensitive quantification of GGT activity in a variety of samples, either colorimetrically (λ = 418 nm) or fluorometrically (Ex/Em = 365/460 nm).


The Alkaline Phosphatase Assay Kits provide highly sensitive, simple and high throughput-compatible assays designed to measure ALP activity in cell lysates, cell culture media, plasma, serum and other biological samples. The kits use either p-nitrophenyl phosphate (pNPP, colorimetric kit) or 4-methylumbelliferyl phosphate disodium salt (MUP, fluorometric kit) as phosphatase substrates which turn yellow (λmax= 405 nm) or fluorescent (Ex/Em = 360nm/440nm) when dephosphorylated by ALP.


PromoKine’s Aspartate Aminotransferase Activity Assay Kit provides a rapid, simple, sensitive and reliable test that is suitable for high throughput applications. In a reversible transamination reaction, AST catalyzes the Transfer of an amino Group from aspartate to ⍺-ketoglutarate, forming oxaloacetate and glutamate. The glutamate is detected in a reaction that converts a nearly colorless probe to a colored product (λmax = 450 nm). Absorbance directly correlates to AST activity in the sample.

The  Alanine Aminotransferase Activity Assay Kit also provides a fast, convenient, reliable and highly sensitive method that is compatible to high throughput applications. In this assay, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate,  forming pyruvate and glutamate. The pyruvate is detected in a reaction that converts a nearly colorless probe to both, a colored (λmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm) product. Absorbance and fluorescence directly correlate to ALT activity in the sample.


PromoKine's Albumin Assay Kit is a simple but sensitive high-throughput assay to detect albumin concentrations (as low as 5 µg/0.01 g/dl) in serum. The assay is based on the selective interaction between Albumin and Bromocresol Green (BCG) which does not react with other abundant plasma proteins like IgG. The reaction results in a chromophore that can be detected at 620 nm, and the signal is directly proportional to the amount of albumin present in the serum. 

Our Bilirubin Assay Kit utilizes the Jendrassik-Grof principle to detect bilirubin. Total bilirubin (unconjugated + conjugated) concentration is determined in the presence of a catalyst, where bilirubin reacts with a diazo-salt to form azobilirubin, which absorbs at 600 nm. Direct bilirubin (conjugated) is determined in the absence of the catalyst at 550 nm.


PromoKine's colorimetric and fluorometric Lactate Dehydrogenase Activity Assay Kits are quick, convenient, and sensitive methods to quantify LDH activity in a variety of biological samples such serum, plasma, cells, culture medium, etc. LDH reduces NAD to NADH, which then interacts with a specific probe to produce either a colored product  (colorimetric kit; λmax = 450 nm) or an intense fluorescent product (fluorometric kit; Ex/Em = 535/587 nm).  The absorbance and fluorescence ist directly proportional to LDH activity in the sample and can be measured in a high throughput fashion using multiwell plate readers.

PromoKine’s LDHA Inhibitor Screening Kit has been to detect potential LDHA Inhibitors that might be used in therapy of various disorders such as cancer. In this high-throughput adaptable, fast colorimetric assay, LDHA catalyzes the conversion of lactate to an intermediate that interacts with a probe to develop a color that can be measured at OD450 nm. In the presence of an LDHA specific inhibitor, the reaction is impeded. An LDHA Inhibitor Control is included to compare the efficacy of the sample inhibitors.

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
Gamma Glutamyl Transferase (GGT) Activity Assay Kit I Convenient kit for sensitive and high-throughput adaptable colorimetric determination of the GGT level in a variety of samples 100 assays PK-CA577-K784 click to select country
Gamma Glutamyl Transferase (GGT) Activity Assay Kit II Convenient kit for sensitive and high-throughput adaptable fluorometric determination of the GGT level in a variety of samples 100 assays PK-CA577-K785 click to select country
Alkaline Phosphatase Activity Assay Kit I Fast, convenient and sensitive colorimetric assay for measuring ALP activity in various biological samples 500 assays PK-CA577-K412 click to select country
Alkaline Phosphatase Activity Assay Kit II Fast, convenient and sensitive colorimetric assay for measuring ALP activity in various biological samples 500 assays PK-CA577-K422 click to select country
Aspartate Aminotransferase (AST/SGOT) Activity Assay Kit The colorimetric kit provides a rapid, simple, sensitive and reliable high-throughput adaptable assay for measuring AST/ SGOT activity in various biological samples 100 assays PK-CA577-K753 click to select country
Alanine Aminotransferase (ALT/SGPT) Activity Assay Kit Fast, convenient and sensitive colorimetric/fluorometric assay for measuring ALT activity in various biological samples 100 assays PK-CA577-K752 click to select country
Bilirubin (Total and Direct) Assay Kit Simple, robust and reliable colorimetric detection of both total or conjugated bilirubin in serum samples 100 assays PK-CA577-K553 click to select country
Albumin Assay Kit Simple, sensitive, and high-throughput adaptable colorimetric assay that detects albumin concentrations in serum 100 assays PK-CA577-K554 click to select country
Lactate Dehydrogenase Activity Assay Kit I Fast, convenient and sensitive colorimetric assay for measuring LDH activity in various biological samples 500 assays PK-CA577-K726 click to select country
Lactate Dehydrogenase Activity Assay Kit II Simple and highly sensitive fluorometric assay for measurement of LDH activity in various tissues and cells 500 assays PK-CA577-K730 click to select country
Lactate Dehydrogenase A Inhibitor Screening Kit Fast, convenient and sensitive colorimetric assay for screening/characterizing/studying potential inhibitors of Lactate Dehydrogenase A 100 assays PK-CA577-K492 click to select country