PromoFectin Non-liposomal Transfection

PromoFectin is a series of reagents for highly efficient and reproducible transfection of a variety of adherent and non-adherent cells with nucleic acids. PromoFectin non-liposomal transfection reagents come either as a broad-range reagent (PromoFectin) as well as specialized reagents for specific cell types such as HUVECs, hepatocytes, and macrophages/monocytes.
For siRNA, mRNA, miRNA and oligonucleotides, we recommend our PromoFectin-siRNA.

Outstanding non-liposomal transfection reagents

PromoFectin provides highly effective and reliable delivery of nucleic acids into a variety of cell types, including many hard-to-transfect cell lines and primary cells such as human endothelial cells, fibroblasts and hepatocytes. It consists of a proprietary non-liposomal, cationic polymer that is stable, water-soluble and free of any components of animal origin. In contrast to many liposome-based transfection reagents, it shows very low toxicity and is therefore ideally suited even for transfection of very sensitive cells yielding optimal transfection efficiencies and gene expression. Transfection with PromoFectin typically requires no medium change and it can be used for highly effective delivery of plasmid DNA as well as oligonucleotides and siRNA into adherent and non-adherent cells. It compacts DNA into positively-charged, stable and protective complexes which bind to negatively-loaded cell-surface residues and enter the cell by endocytosis. In the endosome, PromoFectin acts as a "proton sponge", buffering the endosomal pH that leads to pH inhibition of endosomal nucleases and ensures stability of the complexes. Continuous proton influx leads to osmotic swelling and rupture of the endosome, allowing a very rapid and almost complete release of the nucleic acids into the cytosol. Moreover, PromoFectin also favors DNA trafficking to the cell nucleus - a property that is particularly useful for transfection of slow-dividing or resting cells.

Cell transfection using PromoFectin results in high delivery of nucleic acids (also into hard-to-transfect cell types), extremely low toxic effects (cell stress) and highly efficient intracellular release of nucleic acids due to the unique "Proton Sponge Effect".
Fast and easy transfection procedure that works with adherent and suspension cells in medium with or without serum.
  • superior transfection efficiencies with many cell types
  • lowest cytotoxicity with most cell lines - gentle to sensitive cells
  • suited for stable and transient transfections
  • broad cell-line spectrum - ideally suited also for primary cell transfection
  • simple, straightforward and time-saving protocol
  • compatible with serum and antibiotics
  • remarkably reproducible results
  • ideal for virus production
  • cell-specific versions for endothelial cells (e.g. HUVEC or HDMEC), macrophages and hepatocytes available
Primary human HUVECs transfected with a GFP reporter plasmid using PromoFectin-HUVEC
Primary human HDMECs transfected with a GFP reporter plasmid using PromoFectin-HUVEC
Primary human NHDFs transfected with a GFP reporter plasmid using PromoFectin

Transfection results with primary human endothelial cells (HUVEC and HDMEC) as well as primary human fibroblast cells (NHDF) using PromoFectin/PromoFectin-HUVEC and well-known, leading transfection reagents dedicated for primary cell transfection. Cells were transfected with a luciferase reporter vector. The appropriate transfection protocols recommended by the respective manufacturers were followed. All transfections were performed in triplicate and values were normalized to 1 mg protein.