MATra Protocols & Specifications
Easy Protocol
Example with MATra-A Reagent
Dilute nucleic acid in medium
Add magnetic nanoparticle (MATra-A Reagent)
Incubate 20 - 30 minutes
Add medium to adherent cells (2 - 4 x 105 cells)
Add nucleic acid/nanoparticle solution
Place culture plate on magnet plate
Incubate 15 minutes
Remove magnet plate
Make medium change (recommended for sensitive cell types - e.g. primary cells)
Note: the loading of MATra-A Reagent with nucleic acids has to be performed under serum-free conditions while the nucleic acids loaded MATra-A magnetic particles can be applied to the cells in the presence of serum
MATra Specifications
MATra is applicable at different phases of cell confluence, however in general we recommend 60 - 80% confluence (in some systems a higher visual confluence may result in higher Magnet Assisted Transfection rates).
MATra is non-toxic at recommended amounts in most cell systems. However, if higher MATra/nucleic acid amounts are used, a medium change is recommended after 1 - 2 h. For transfection of very sensitive cells (e.g. some primary cells), we recommend medium change directly after the transfection procedure and adjusting the amount of the MATra-A/nucleic acid concentration individually. Reducing the MATra-A concentration might result in better cell viability without decreasing transfection efficiency significantly.
The MATra technology is compatible with robots for high-throughput transfections!
Low Vector Doses
- Magnet Assisted Transfection and lipofection in comparison. Luciferase expression was assayed after 24 hours.
Assay Formats
Formats | Volume of MATra-A reagent recommended [µl] | Transfections per vial (200 µl vial)* |
96 well plate | 0.1 | 2000 |
48 well plate | 0.3 | 667 |
24 well plate | 0.6 | 333 |
12 well plate | 1.2 | 167 |
6 well plate | 3 | 67 |
60 mm dish | 6.6 | 30 |
100 mm dish | 17.2 | 12 |
T-75 flask | 23.5 | 9 |
26x26 cm plate | 156 | 1** |