Products

Matrix Metalloproteinase (MMP) Activity & Inhibitor Screening Kits

Matrix metalloproteinases (MMPs) are a group of secreted, zinc-dependent endopeptidases that are responsible for the degradation of most extracellular matrix (ECM) proteins - including collagens, elastins, gelatin, matrix glycoproteins and proteoglycan - during growth, normal tissue development and turnover, organogenesis, as well as different disease processes. MMPs are regulated by hormones, growth factors and cytokines and are differentially expressed in tumor cells. Some MMPs  are implicated in cancer progression and metastasis (e.g. MMP-2, MMP-3, MMP-9 and MMP-14) while MMP-1 is thought to be important in rheumatoid arthritis and osteoarthritis. Thus, MMP inhibitors are promising candidates for treatment of cancer and other diseases.

PromoKine's fluorometric MMP Inhibitor Screening Kits provide a rapid, simple, sensitive, and reliable high throughput screening assay for MMP inhibitors. They use a a specific FRET substrate that is hydrolyzed by the MMPs to release a quenched fluorescent group or cleave a synthetic MCA-based peptide substrate (MMP-14 Inhibitor Screening Kit) to release free MCA (7-methoxycoumarin-4-acetic acid). In presence of potent MMP Inhibitors, the clevage of substrate will be inhibited or stopped, resulting in decrease or total loss of the fluorescence signal. For comparison of the relative efficacy of test inhibitors, a control inhibitor is included in the kits.

The MMP-3 Activity Assay Kit provides a rapid, simple, sensitive and high throughput-compatible method to quantify MMP-3 activity. It  also uses a specific FRET substrate which is hydrolyzes by MMP-3 to release the quenched fluorescent group MCA, which can be detected fluorometrically. 

See our website for more MMP products (recombinant proteins, antibodies and ELISAs).

Product Name Description Size Catalog Number Information Prices
MMP-3 Activity Assay Kit Fluorometric assay for measuring MMP-3 activity in various biological samples 100 assays PK-CA577-K783 click to select country
MMP-1 Inhibitor Screening Kit Fluorometric assay to easily screen/study/charecterize MMP-1 inhibitors 100 assays PK-CA577-K794 click to select country
MMP-3 Inhibitor Screening Kit Fluorometric assay to easily screen/study/charecterize MMP-3 inhibitors 100 assays PK-CA577-K793 click to select country
MMP-9 Inhibitor Screening Kit Fluorometric assay to easily screen/study/charecterize MMP-9 inhibitors 100 assays PK-CA577-K844 click to select country
MMP-14 Inhibitor Screening Kit Fluorometric assay to easily screen/study/charecterize MMP-14 inhibitors 100 assays PK-CA577-K846 click to select country