Products

HAT and pCAF Activity Assay & Inhibitor Screening Kits

Histone acetyltransferases (HATs) have been implicated in playing a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.

The colorimetric HAT Activity Assay Kit I offers a convenient, non-radioactive system for a rapid and sensitive, spectrophotometric detection of HAT activity in mammalian samples. The detection can be continuous and is therefore suitable for kinetic studies.

PromoKine’s fluorometric HAT Activity Assay Kit II utilizes Acetyl-CoA and H3 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer to generate a product that is detected fluorometrically at a high sensitivity.

The ultra-sensitive fluorometric HAT Activity Assay Kit III can detect HAT activity equivalent to as low as 1.5 µU of p300 in a variety of samples.

pCAF also known as K (lysine) acetyltransferase 2B (KAT2B), possesses intrinsic histone acetyltransferase (HAT) activity. pCAF contributes to transcriptional activation by acetylating chromatin and transcription factors through its HAT activity. Deregulated HAT activity plays an important role in various cancers and thus pCAF inhibitors have potential applications in cancer therapy.
PromoKine's pCAF Inhibitor Screening Kit provides a simple, sensitive, and rapid tool to screen potential pCAF inhibitors.

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
HAT Activity Assay Kit I Detects HAT activity by colorimetric method in mammalian samples 100 assays PK-CA577-K332 click to select country
HAT Activity Assay Kit II Highly sensitive fluorometric detection of HAT activity in mammalian samples 100 Assays PK-CA577-K334 click to select country
HAT Activity Assay Kit III Ultra-sensitive fluorometric detection of HAT activity in mammalian samples 100 Assays PK-CA577-K336 click to select country
pCAF Inhibitor Screening Kit Fast, simple and sensitive fluorometric assay for screening/studying/characterizing potential inhibitors of pCAF. 100 Assays PK-CA577-K345 click to select country
HAT protein (P/CAF, active) Recombinant, active histone acetyltransferase (HAT) 100 µg PK-RP577-1137-100 click to select country