DNA Damage Detection Kit

Different physiological and environmental factors such as chemical induced damage and cell aging are causing DNA lesions. AP sites (apurinic/apyrimidinic sites) are one of the major types of DNA lesions formed during the process of base excision and repair of oxidized, deaminated, or alkylated bases. It has been estimated that about 2x105 base lesions are generated per cell per day. For this reason, the level of AP sites in cells can be a good indicator of DNA lesions and repairs.
The DNA Damage Detection Kit utilizes the ARP (Aldehyde Reactive Probe) reagent that reacts specifically with the aldehyde group in the open ring form of the AP sites. After treating DNA containing AP sites with ARP reagents, AP sites are tagged with biotin residues, which can be quantified using an avidin-biotin assay followed by a colorimetric detection. The kit provides the necessary reagents for convenient determination of abasic sites in purified DNA sample in 96-well plate format.

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
DNA Damage Detection Kit For convenient determination of DNA damage (abasic sites) in sample DNA in 96-well plate format 25 assays PK-CA577-K253 click to select country