Assays for Alzheimer's Disease Research

Alzheimer’s disease is a chronic neurodegenerative disorder that is most probably caused by accumulation of amyloid plaques found in the brains of patients suffering this condition. Plaques are mainly composed of Beta amyloid (Aβ) peptides: Aβ (1-40) and Aβ (1-42). Aggregated Aβ (1-42) is considered a validated biomarker for diagnosis of Alzheimer’s disease. Therefore, screening Aβ42 Ligands that could prevent aggregation is critical for developing potential therapeutic treatments.

β-Secretase (BACE) is a membrane-bound aspartyl protease that cleaves the amyloid precursor protein and is consequently an excellent target for anti-amyloid therapy in the treatment of Alzheimer's disease. Finding inhibitors of β-secretase is one of the major goals of Alzheimer's disease drug development.

PromoKine's β-Secretase Fluorometric Assay Kit provides a convenient, non-radioactive system for detecting β-Secretase activity in biological samples. The kit provides active β-Secretase as positive control, β-Secretase inhibitor as negative control, optimized peptide substrate (conjugated to two reporter molecules), and buffers for convenient measurement of β-Secretase activity in mammalian samples. β-Secretase inhibitors are also available separately. 

In the β-Secretase Inhibitor Screening Kit, human BACE1 cleaves a quenched substrate, specific for BACE1, generating a product with high fluorescence that can be measured at Ex/Em= 435/500 nm. In the presence of BACE1 Inhibitor (a control is included in this kit), the enzymatic cleavage is impeded.  The assay can be used to screen for new inhibitor compounds. It is high-throughput adaptable and can be completed in less than 1 hour.

In PromoKine’s Beta Amyloid 1-42 (Aβ42) Ligand Screening Kit, a dye binds to the beta sheets of an aggregated amyloid peptide resulting in an intense fluorescent product (Ex/Em: 440/490 nm). In the presence of an Aβ42 ligand, this reaction is impeded/abolished resulting in decrease or total loss of fluorescence. This simple, reliable and high throughput-adaptable assay is useful in screening of Aβ42 ligands for developing potential therapeutic agents against Alzheimer’s disease. 

Congo Red and DCDAPH can be used to stain Amyloid Protein and Aβ plaques in brain sections.

Product Name Description Size Catalog Number Information Prices
Beta-Secretase Fluorometric Assay Kit Fluorometric assay for convenient detection of b-secretase in biological and purified samples 100 assays PK-CA577-K360 click to select country
Beta-Secretase Inhibitor Screening Kit Highly sensitive, high throughput-compatible fluorometric assay for screening potential BACE1 inhibitors 100 assays PK-CA577-K720 click to select country
Beta Amyloid 1- 42 (Aβ42) Ligand Screening Assay Kit Fluorometric assay suitable for high-throughput compatible, quick and easy screening for Aβ42 ligands 100 assays PK-CA577-K570 click to select country
Congo Red (high purity grade) Congo Red has been used in microscopy for staining elastic fibers and bacteria. Amyloid protein, an abnormal protein aggregate associated with various pathologies, is commonly detected in tissue with Congo Red. 100 mg PK-CA707-80028 click to select country
DCDAPH NIR fluorescent probe that has proved to have affinity to Aβ plaques in fluorescent staining of brain sections as well as in an in vivo binding assay using Aβ1-42 aggregates. 5 mg PK-CA707-80030 click to select country
beta-Secretase 1 (BACE-1), human, recombinant Recombinant Human beta-Secretase 1 (BACE-1) 5 µg PK-RP577-7609-5 click to select country
beta-Secretase 1 (BACE-1), human, recombinant Recombinant Human beta-Secretase 1 (BACE-1) 50 µg PK-RP577-7609-50 click to select country
Beta-Secretase Inhibitor I Protein inhibitor of the amyloid precursor protein (APP) beta-secretase 1 mg PK-CA577-7501-1 click to select country
Beta-Secretase Inhibitor II A potent, cell permeable, and reversible inhibitor of beta-secretase 1 mg PK-CA577-7502-1 click to select country