GFP Reporter Assay Kit

GFP (Green Fluorescent Protein) is a fluorescent protein originally isolated from the jellyfish Aequorea victoria. GFP and its enhanced variant EGFP have been widely used as reporter proteins for in vivo monitoring of gene expression in a variety of cell types and organisms. Since GFP requires no additional substrates or cofactors, GFP fluorescence can easily be detected using a fluorescence microscope. However, most imaging studies of GFP are qualitative. Quantitative analyses of GFP expression levels in cells or tissues are more informative and have wider applications. The GFP Reporter Assay Kit has been designed for use in a 96-well micro-plate format (Ex/Em = 488 nm/507nm). Cells or tissues can be homogenized directly in the provided GFP assay buffer and the supernatant of the lysed cells/tissues is transfered to the microwell plates. The amount of GFP is determined by comparing its fluorescence with that of a GFP standard. The kit can detect a wide range of GFP concentrations (0.01-10 μg/ml). Furthermore, a GFP quench solution is provided for determining autofluorescence levels of cell or tissue extracts.

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Product Name Description Size Catalog Number Information Prices
GFP Reporter Assay Kit For Quantitation of GFP in 96 micro-plate format 100 assays PK-CA577-K815 click to select country