3D Cell Culture Viability Assay Kit

Quantification of viable cell numbers is an indispensable tool in in vitro screening in many biological and pharmacological studies. Calcein-AM is a non-fluorescent compound that easily penetrates intact and live cells and does fluoresce brightly green once the AM group is cleaved off by intracellular esterases. It has been widely used to assess cell viability and proliferation in cell biology research. However, with the use of 3D matrices, some protease-based dissociation methods do not completely dissolve the matrices and cell aggregates, which can distort results of quantitative in vitro assays such as viability assessment.

PromoKine‚Äôs 3D Cell Culture Viability Assay Kit provides an easy-to-use, high-throughput and sensitive method for screening, characterizing and studying cell viability, and cytotoxicity in three-dimensional cell cultures. The standardized fluorometric method reliably quantifies viable cells detecting as low as 50 viable cells per well. The measured fluorescence intensity at Ex/Em = 485/530 nm is directly proportional to the number of viable cells. The complete kit comes with an optimized and gentle non-enzymatic dissociation solution for the recovery of viable and dead cells from spheroids in matrices and scaffolds.     

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Product Name Description Size Catalog Number Information Prices
3D Cell Culture Viability Assay Kit Provides a standardized fluorometric method for sensitive quantification of viable cells in 3D cell culture. 100 assays PK-CA577-K948 click to select country