3D Cell Culture Matrices

PromoKine offers a range of 3D Cell Culture Matrix Kits, including Basement Membrane Extract (BME, animal-based), Alginate Hydrogel (plant-based), proprietary Duo-Matrix and Dry Scaffold (natural polymers, animal-free) to meet the needs and requirements of various research fields. Our 3D Culture Matrix Kits provide a standardized, yet user friendly and adaptable to high-throughput strategy for setting up spheroid/organoid formations, 3D cell cultures and pharmacological studies to screen and characterize compounds in a more natural 3D environment.

PromoKine’s 3D Cell Culture Matrix Kit (BME) contains a commonly used cell-adhesive matrix with a microporous structure which is derived from animal sources. It provides a very good scaffold for cells to grow in a 3D fashion - enabling mimicking of cellular processes and responses much more similar to natural in vivo environments than 2D cell culture. BME is a more naturally relevant matrix and for example well suited for modeling the morphological effects of early oncogenesis on three-dimensional microenvironments. However, as it is sourced from animals there is certain lot to lot variability and growth factor contamination. PromoKine's BME is a growth factor-reduced and endotoxin-free matrix.

PromoKine’s 3D Cell Culture Matrix Kit (Alginate Hydrogel) is based on alginate, an anionic polysaccharide derived from the cell walls of algae. It provides a nanoporous matrix which lacks cell adhesion sites as well as growth hormones and other ECM proteins. Hence, it is a more controlled environment which is also easy to handle and preferred by certain cell types. As it solidifies to a gel in the presence of calcium ions and liquefies in the presence of calcium-chelating agents, it is an ideal scaffold for culturing of cells in three dimensions and conveniently harvesting these cultured cells. It supports successful 3D cell culture of different cell types including e.g. tumor cells.

Our proprietary 3D Cell Culture Matrix Kit (Duo-Matrix) is a proprietary blend of an animal- and a non-animal-derived matrix that provides a more dynamic microenvironment as opposed to a single component matrix. Thus, it is a preferred environment for modeling cell growth, migration and differentiation.

PromoKine’s ready-to-use 3D Cell Culture Scaffold Kit provides a standardized and high-throughput adaptable strategy with a ready-to-use 96-well microtiter plate that contains proprietary 3D scaffolds for setting up spheroid formations, 3D cell cultures, and pharmacological studies, with an optimized scaffold dissociation method.