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3D Cell Culture Harvesting Kit

Isolating intact cells in 3D cell culture from the extracellular matrix proteins comprising the 3D matrices in order to use them for cell passaging or biochemical and molecular analysis can be very difficult. While proteases (such as trypsin and accutase) are commonly used to degrade these matrices, this is not recommended in some cases where cells are sensitive to protease digestion as these proteases may modify cell surface and/or cellular proteins and signaling, thus altering physiological assessments. Further, some protease-based dissociation methods do not completely dissolve the matrices.

PromoKine’s 3D Cell Culture Harvesting Kit provides an optimized and standardized saline-based solution for a simplified non-enzymatic isolation of cells and spheroids from matrices (especially for PromoKine’s 3D Cell Culture Kits: PK-CA577-K517, -K518, -K519) with high viability rates for subsequent biochemical, protein and cell-based analysis. This kit provides reagents for extracting cells and/or cell aggregates from 3D cell cultures for biochemical analysis, as well as reagents for the preparation of lysates for immunoblotting.

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
3D Cell Culture Harvesting Kit Provides an optimized and standardized saline-based solution for the isolation of cells and spheroids from matrices with high viability rate for subsequent biochemical, protein and cell-based analysis. 100 samples PK-CA577-K982 click to select country