Products

Products for Gel Electrophoresis

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Mycoplasma-EX Wipes

  • Cleaning and disinfecting laboratory surfaces at the same time
  • Works efficiently against mycoplasmas, fungi, bacteria, and viruses
  • Contains an alcohol-based, non-corrosive and non-carcinogenic solution with a reagent that kills mycoplasmas effectively and fast
  • Simple-to-use wipes in a convenient storage box

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Products for Cell Analysis and Apoptosis

PromoKine has extended its innovative cell biology product line and is now offering many new reagents and kits for cell analysis and Apoptosis e.g.;

Cell Proliferation Kit I (CFSE) for flow cytometry and fluorescence microscopy providing a convenient and sensitive way to determine cell proliferation, cell division and cell fusion. More information

DiO/DPA Membrane Potential Detection Kit for sensitive detection of cytoplasmic membrane potential changes using FRET technology. More information

RTU DNA Ladders and FluoDNA Gel Stain. More information

Prestained RTU Protein Ladders, FluoProtein Gel Stain and GelQuick Protein Gel Stain/Destaining KitMore information

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Animal-free Cytokines & Growth Factors

In addition to its wide range of recombinant cytokines, growth factors, receptors and CD markers from human, mouse and rat, PromoKine now also offers a selection of recombinant cytokines and growth factors that have been produced plants (barley seed endosperm or tobacco) at high isogenic quality.

  • No proteolytic, pyrogenic, or inflammatory activity
  • Free of bacterial, viral and prion infectious agents as well as contaminating molecules such as endotoxins (bacterial expression host) or cytokines, chemokines and growth factors (mammalian expression host)
  • No serum carryover that may cause unwanted effects (such as proliferation)
  • Proper eukaryotic folding and post-translational modification (e.g. glycosylation) that increases biological activity and stability  
  • More than 20 cytokines and growth factors available (e.g. Activin A, BAFF, BMP-2, BMP-7, EGF, FGF-2, Flt3-Ligand, GM-CSF, LIF, M-CSF, Myosatin, diverse Interleukins, IFN-alpha, IFN-gamma, TGF-beta, TNF-alpha, VEGF)

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Cytokines & Growth Factors for Stem Cell Research

Among PromoKine's wide range of cytokines and growth factors (human, mouse and rat) you will find numerous cytokines and growth factors important in Stem Cell Research. These recombinant proteins have been produced in E. coli, yeast, insect, mammalian or plant cells and show high activity and purity. 

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Wide range of ELISA Kits

  • for sensitive and reliable quantitation of specific targets important in cell signaling (e.g. cytokines), apoptosis, diseases and metabolism
  • ready-to-use kits with comprehensive and user-friendly protocols
  • also available: economically priced ELISA Development Kits!

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Western blot and immunohistochemical analysis of human AIF.

Antibodies for Cell Biology

Find many new antibodies, e.g. for:

as well as for:

Our monoclonal and polyclonal antibodies comply with the highest quality standards and are useful for many different applications. More information

See also:

Labeled Secondary Antibodies
Protein & Antibody Labeling Kits 


PromoFectin-Macrophage

  • superior transfection efficiencies
  • extremely low cytotoxicity                              
  • convenient and fast protocol
  • compatible with serum and antibiotics
  • reproducible results

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Labeling of cell structures using red-fluorescing PromoFluor-647 dye (illustration kindly provided by Covalys Biosciences AG).

PromoFluor: New Superior Fluorescence Dyes

  • strong absorption
  • high photostability - reduced photobleaching
  • good pH insensitivity and water solubility
  • high fluorescence quantum yield - optimal brightness 
  • same quality but better pricing than many commonly used Alexa Fluor® or Cy™ dyes!
  • spans the whole visible and infrared light spectrum (350-840 nm) 
  • Now available: PromoFluor 532 with yellow-fluorescent emission

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PromoFluor AntiFade Reagent

  • stabilizes fluorochromes and reduces photobleaching
  • enhances fluorescent signals
  • perfect mounting medium: conserves and protects your precious experimental samples

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Gene Cloning, Expression & Silencing

Versatile vector systems for convenient cloning and efficient expression of genes

PromoKine now offers a selection of reporter vectors (with e.g. luciferase, β-Gal and GFP reporter genes) and fusion cloning vectors as well as vectors that have been optimized for over-expression of target genes. Those vectors are based on a high-level constitutive CMV promoter and are suited for transient as well as stable gene expression. Due to their small size and convenient multiple cloning sites they are ideal for rapid and efficient cloning and enable optimal cell transfection results. More information

PromoKine also provides PCR-based kits for fast, convenient and efficient gene cloning and high-level gene expression in mammalian cells. More information

Diverse vectors and kits for gene silencing experiments are also available. The vector systems have been designed for efficiently expressing siRNAs in mammalian host cells (with or without a GFP tag) while the kits have been developed for synthesizing the desired siRNA molecules to be transfected into host cells by PCR or T7 in vitro transcription. More information

In addition, the innovative StarGate Technology allows you to easily clone your gene of interest into an Entry Vector which then is the origin (Donor Vector) for transfering it conveniently and fast to various Acceptor Vectors providing different features (e.g. different combinations of promoters, affinity and reporter tags). Thus, the gene of interest can be fused with many different genetic surroundings and can be expressed in different host systems (mammalian and insect cells, E. coli and yeast) without the need of cumbersome and time-consuming subcloning procedures. StarGate is the method of choice to optimize expression of your target gene and purification of the respective gene product. The StarGate technology comprises the StarGate Combinatorial Cloning System as well as the StarGate Mutagenesis System and the StarGate Fusion Cloning System.  More information

 


MATra: Magnet-Assisted Transfection

  • highest transfection efficiencies
  • very low cytotoxicity
  • low costs compared e.g. with electroporation-based methods

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