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Gene Cloning, Expression & Silencing

Versatile vector systems for convenient cloning and efficient expression of genes

PromoKine now offers a selection of reporter vectors (with e.g. luciferase, β-Gal and GFP reporter genes) and fusion cloning vectors as well as vectors that have been optimized for over-expression of target genes. Those vectors are based on a high-level constitutive CMV promoter and are suited for transient as well as stable gene expression. Due to their small size and convenient multiple cloning sites they are ideal for rapid and efficient cloning and enable optimal cell transfection results. More information

PromoKine also provides PCR-based kits for fast, convenient and efficient gene cloning and high-level gene expression in mammalian cells. More information

Diverse vectors and kits for gene silencing experiments are also available. The vector systems have been designed for efficiently expressing siRNAs in mammalian host cells (with or without a GFP tag) while the kits have been developed for synthesizing the desired siRNA molecules to be transfected into host cells by PCR or T7 in vitro transcription. More information

In addition, the innovative StarGate Technology allows you to easily clone your gene of interest into an Entry Vector which then is the origin (Donor Vector) for transfering it conveniently and fast to various Acceptor Vectors providing different features (e.g. different combinations of promoters, affinity and reporter tags). Thus, the gene of interest can be fused with many different genetic surroundings and can be expressed in different host systems (mammalian and insect cells, E. coli and yeast) without the need of cumbersome and time-consuming subcloning procedures. StarGate is the method of choice to optimize expression of your target gene and purification of the respective gene product. The StarGate technology comprises the StarGate Combinatorial Cloning System as well as the StarGate Mutagenesis System and the StarGate Fusion Cloning System.  More information

 


Products for Cell Analysis and Apoptosis

Fast, convenient and highly sensitive

PromoKine has extended its innovative cell biology product line and is now providing many new reagents and kits for:

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High quality and economically priced reagents and kits for convenient, sensitive and reliable cell analysis and apoptosis research.



Western blot and immunohistochemical analysis of human AIF.

Antibodies for Cell Biology

High quality - low price

Find many new antibodies, e.g. for:

as well as:

Our monoclonal and polyclonal antibodies comply with the highest quality standards and are useful for many different applications.

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See also:

Labeled Secondary Antibodies

Protein & Antibody Labeling Kits 

Antibody Purification Kits

 

 

 


Primary hepatocytes transfected with a GFP reporter plasmid using PromoFectin-Hepatocyte.

PromoFectin

Highly efficient transfection of hard-to-transfect, sensitive cells

  • superior transfection efficiencies
  • extremely low cytotoxicity - gentle to fragile cells such as primary cells                                                                   
  • broad cell-line spectrum
  • convenient and fast protocol
  • compatible with serum and antibiotics
  • reproducible results
  • optimized protocols for HTS applications and protein production available
  • cell-specific PromoFectin-reagents (e.g. for hepatocytes, endothelial cells, insect cells or distinct cell lines) and reagents for delivery of siRNA and proteins/antibodies are also available

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MATra: Magnet-Assisted Transfection

Optimize your transfection results with common cell lines - and particularly with difficult-to-transfect cells such as primary cells!

  • highest transfection efficiencies
  • very low cytotoxicity
  • low costs compared e.g. with electroporation-based methods

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Labeling of cell structures using red-fluorescing PromoFluor-647 dye (illustration kindly provided by Covalys Biosciences AG).

PromoFluor: New Superior Fluorescence Dyes

Highly efficient labeling of biomolecules (proteins, antibodies, nucleic acids)

  • strong absorption
  • high photostability - reduced photobleaching
  • good pH insensitivity and water solubility
  • high fluorescence quantum yield - optimal brightness 
  • same quality but better pricing than many commonly used Alexa Fluor® or Cy™ dyes!

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PromoFluor AntiFade Reagent

  • stabilizes fluorochromes and reduces photobleaching
  • enhances fluorescent signals
  • perfect mounting medium: conserves and protects your precious experimental samples

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Mycoplasma Detection and Elimination

PromoCell now offers a range of excellent and cost-effective mycoplasma safety tools for detection and elimination of different mycoplasma species in cell culture. This includes highly sensitive and specific detection kits based on conventional or Real-Time PCR as well antibiotic and non-antibiotic reagents for complete and reliable elimination of mycoplasma contamination. Our PCR Mycoplasma Test Kits contain all reagents as well as first-class Taq polymerase and internal standards required to perform convenient, rapid and reproducible conventional PCRs - that can be analyzed by agarose gel electrophoresis - or Real-Time PCRs using cyclers from different manufacturers. Our Mycoplasma-EX and BIOMYC ready-to-use solutions are highly efficient resulting in 100% elimination of mycoplasma contaminating your cell culture - but do not harm your precious cells. Mycoplasma-EX Spray and Pharmacidal Spray that can be used to clean and disinfect laboratory surfaces and apparatuses complete our anti-mycoplasma arsenal.
New: Improved version of the Real-Time PCR Mycoplasma Test Kit available now!

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