Products

PCR Mycoplasma Test Kit I/RT

The PCR Mycoplasma Test Kit I/RT utilizes quantitative, real-time PCR (qPCR). It shows excellent sensitivity (detects approximately 10 mycoplasmas per sample volume) and specificity and allows to obtain reliable PCR results extremely fast (in "real time"). Thus, the kit is suited particularly well for routine testing/screening for mycoplasma contamination using cell culture supernatants. It contains mycoplasma-specific probes and comes with Inhibition Control DNA as well as Positive Control DNA. Defined DNA standards for quantitative Real-Time PCR are available separately.

The kit detects a wide range of mycoplasma species and is compatible with any Real-Time PCR instrument able to detect the fluorescence dyes FAM™ and HEX™. The complete detection procedure can be performed within 3 hours.

Background

Mycoplasmas are specifically detected by amplifying a highly conserved 23S rRNA coding region in the mycoplasma genome. This mycoplasma-specific amplification is detected at 520 nm (FAM™ channel).The kit includes a lyophilized, complete Master Mix containing primer and FAM™ labeled probes which allow the specific detection of all Mollicutes species so far described as contaminants of cell cultures and media components. Eukaryotic DNA is not amplified by this primer/probe system.
False negative results due to PCR inhibitors or improper DNA extraction are detected by the Internal Amplification Control which can be added to the PCR master mix. The amplification of the internal amplification control is detected at 560 nm (HEX™ channel).

Kit Components PCR Mycoplasma Test Kit I/RT; Variant C

  • Primer&Probes Mix: containing mycoplasma specific primer set; nucleotides dATP, dCTP, dGTP and dUTP; Taq DNA Polymerase; (lyophilized master mix; for 25 or 50 reactions)
  • Inhibition Control Spike: containing artificial DNA fragments/plasmid DNA (lyophilized; non-infectious; for at least 25 or 50 reactions)
  • Positive Control Spike, containing artificial DNA-fragments/plasmid DNA (lyophilized; non-infectious; for at least 25 or 50 reactions)
  • Rehydration/Reaction Buffer
  • DNA-free Water
  • Instruction manual

Mollicutes species detectable with PCR Mycoplasma Test Kit I/RT, Variant C

Based on positive testing

Based on sequence alignments

Based on sequence alignments

Mycoplasma arginini

Mycoplasma agalactiae

Mycoplasma hyopharyngis

Mycoplasma arthritidis

Mycoplasma alligatoris

Mycoplasma hyopneumoniae

Mycoplasma fermentans

Mycoplasma alvi

Mycoplasma hyosynoviae

Mycoplasma gallisepticum

Mycoplasma amphoriforme

Mycoplasma iguanae

Mycoplasma genitalium

Mycoplasma anatis

Mycoplasma imitans

Mycoplasma hominis

Mycoplasma bovis

Mycoplasma mobile

Mycoplasma hyorhinis

Mycoplasma canis

Mycoplasma ovipneumoniae

Mycoplasma orale

Mycoplasma columbinum

Mycoplasma pulmonis

Mycoplasma penetrans

Mycoplasma conjunctivae

Mycoplasma testudineum

Mycoplasma pneumoniae

Mycoplasma crocodyli

Mycoplasma testudinis

Mycoplasma salivarium

Mycoplasma flocculare

 

Mycoplasma synoviae

 

 

Acholeplasma laidlawii

 

 

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
PCR Mycoplasma Test Kit I/RT; Variant C Test Kit for Real-Time qPCR 25 assays PK-CA91-3025C click to select country
PCR Mycoplasma Test Kit I/RT; Variant C Test Kit for Real-Time qPCR 50 assays PK-CA91-3050C click to select country
Mycoplasma fermentans DNA Standard (for qPCR) Defined quantification standard for the Real-Time PCR Mycoplasma Test Kits (1.000.000 genomes/µl) 100 µl PK-CA91-0117 click to select country
Mycoplasma orale DNA Standard (for qPCR) Defined quantification standard for the Real-Time PCR Mycoplasma Test Kits (1.000.000 genomes/µl) 100 µl PK-CA91-0112 click to select country


*Please note: Kits PK-CA91-3025A/PK-CA91-3050A and PK-CA91-3025B/PK-CA91-3050B have been discontinued and replaced by the universal PK-CA91-3025C/PK-CA91-3050C kits.