Products

BIOMYC Antibiotic Solutions

BIOMYC-1 and BIOMYC-2

BIOMYC-1 is a solution based on the antibiotic tiamutin which is produced by the fungus Pleurotus mutilus. BIOMYC-2 is a solution based on the antibiotic minocycline, which is a tetracycline derivative.

These two antibiotics have been shown to be very effective in eliminating the mycoplasma species frequently present in contaminated cell cultures and are generally used sequentially in combination. Following our protocol, the mycoplasmas do not develop resistance to these antibiotics - a common occurrence with other antibiotic treatment methods. The treatment is based on alternating use of the two antibiotics: four days with tiamutin followed by three days with minocycline, and repeating the cycle 2-3 times. On completion of the treatment, the cells should be grown on media without antibiotics for at least two weeks and then re-tested for the presence of mycoplasmas. If the culture test is positive, the course of treatment should be repeated.

Instructions for Use

    • Do not use the two solutions together, but rather sequentially.
    • Add 1 ml BIOMYC-1 to 100 ml medium, and maintain the contaminated cells in this mixture for 4 days. Any fresh medium added should also contain BIOMYC-1.
    • After 4 days, add 1 ml BIOMYC-2 to 100 ml fresh medium, and maintain the cells in this second mixture for 3 days.
    • The above, together, are considered as one treatment cycle. It may be necessary to repeat this cycle 2-3 times.
    • During the process, the cells can be tested for mycoplasma contamination, and results can then be used to shorten the process when possible.

BIOMYC-3

BIOMYC-3 is based on the antibiotic ciprofloxacin, which is a member of the fluoroquinolone group and inhibits synthesis of the enzyme DNA gyrase which is responsible for supercoiling in bacterial DNA. Many mycoplasma species have been found to be sensitive to BIOMYC-3, including A. laidlawii, M. orale, M. hyorhinis, M. fermentans, and M. arginini. These species are responsible for most mycoplasma contamination in cell culture. At the concentrations recommended for use, no cytotoxic effects have been found so far, and the treatment is quite easy to perform.

The treatment consists of adding the antibiotic to the growth medium for 12 days, and changing the medium every three days. On completion of the treatment, the cells should be grown on media without antibiotics for at least two weeks and then re-tested for the presence of mycoplasma. If the culture tests positive, the course of treatment should be repeated. Treatment with ciprofloxacin does not damage or alter the cells. This antibiotic should not be used repeatedly in cell cultures; otherwise, the mycoplasmas are liable to develop resistance to it.

Instructions for Use

    • Add 1 ml BIOMYC-3 to 100 ml medium.
    • Continue the treatment for a total of 14 days, while changing the medium (containing BIOMYC-3) every 2-3 days.
    • Retain the cells in the growth medium for an additional 14 days before re-testing for mycoplasmas.

Reference

Schmitt, K. et al., J. Immunol. Methods, 109:17-25 (1988).

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
BIOMYC-1 Antibiotic Solution (100x) 10 ml PK-CC03-036-1D click to select country
BIOMYC-1 Antibiotic Solution (100x) 20 ml PK-CC03-036-1C click to select country
BIOMYC-1 Antibiotic Solution (100x) 100 ml PK-CC03-036-1B click to select country
BIOMYC-2 Antibiotic Solution (100x) 10 ml PK-CC03-037-1D click to select country
BIOMYC-2 Antibiotic Solution (100x) 20 ml PK-CC03-037-1C click to select country
BIOMYC-2 Antibiotic Solution (100x) 100 ml PK-CC03-037-1B click to select country
BIOMYC-3 Antibiotic Solution (100x) 10 ml PK-CC03-038-1D click to select country
BIOMYC-3 Antibiotic Solution (100x) 20 ml PK-CC03-038-1C click to select country
BIOMYC-3 Antibiotic Solution (100x) 100 ml PK-CC03-038-1B click to select country