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Secondary Detection


Labeled Antibodies

PromoKine offers high-quality secondary (polyclonal) antibodies at an excellent price / performance ratio. These antibodies are conjugated to horseradish peroxidase (HRP), Alkaline Phosphatase (AP), Biotin, Fluorescein, Texas Red or our superior PromoFluor fluorescent dyes. They can be used for different applications such as Western blotting, fluorescence microscopy and flow cytometry.

PromoFluor Labeled Secondary Antibodies

Catalog Number Product Name Description Size Information Add to Cart
PK-AB718-9360 Donkey Anti-Goat antibody (pAb), Fluorescein-coupled Donkey Anti-Goat IgG conjugated to Fluorescein (H&L) 2 mg
PK-AB718-9370 Donkey Anti-Goat antibody (pAb), Texas Red-coupled Donkey Anti-Goat IgG conjugated to Texas Red (H&L) 2 mg
PK-AB718-9170 Goat Anti-Chicken antibody (pAb), Texas Red-coupled Goat Anti-Chicken IgG conjugated to Texas Red (H&L) 2 mg
PK-AB718-9780 Goat Anti-Mouse antibody (pAb), AP-coupled Goat Anti-Mouse IgG conjugated to Alkaline Phosphatase (H&L, minimum cross-reactivity) 1 mg
PK-AB718-9740 Goat Anti-Mouse antibody (pAb), Biotin-coupled Goat Anti-Mouse IgG conjugated to Biotin (H&L, minimum cross-reactivity) 2 mg
PK-AB718-9760 Goat Anti-Mouse antibody (pAb), Fluorescein-coupled Goat Anti-Mouse IgG conjugated to Fluorescein (H&L, minimum cross-reactivity) 2 mg
PK-AB718-9720 Goat Anti-Mouse antibody (pAb), HRP-coupled Goat Anti-Mouse IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity) 2 mg
PK-AB718-9770 Goat Anti-Mouse antibody (pAb), Texas Red-coupled Goat Anti-Mouse IgG conjugated to Texas Red (H&L) 2 mg
PK-AB718-9980 Goat Anti-Rabbit antibody (pAb), AP-coupled Goat Anti-Rabbit IgG conjugated to Alkaline Phosphatase (H&L, minimum cross-reactivity) 1 mg
PK-AB718-9940 Goat Anti-Rabbit antibody (pAb), Biotin-coupled Goat Anti-Rabbit IgG conjugated to Biotin (H&L, minimum cross-reactivity) 2 mg
PK-AB718-9960 Goat Anti-Rabbit antibody (pAb), Fluorescein-coupled Goat Anti-Rabbit IgG conjugated to Fluorescein (H&L, minimum cross-reactivity) 2 mg
PK-AB718-9920 Goat Anti-Rabbit antibody (pAb), HRP-coupled Goat Anti-Rabbit IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity) 2 mg
PK-AB718-9970 Goat Anti-Rabbit antibody (pAb), Texas Red-coupled Goat Anti-Rabbit IgG conjugated to Texas Red (H&L) 2 mg
PK-AB577-6401 Goat Anti-Rabbit IgG, HRP-coupled antibody (pAb) Goat Anti-Rabbit IgG conjugated to Horseradish Peroxidase 0.5 ml
PK-AB718-9580 Goat Anti-Rat antibody (pAb), AP-coupled Goat Anti-Rat IgG conjugated to Alkaline Phosphatase (H&L, minimum cross-reactivity) 1 mg
PK-AB718-9520 Goat Anti-Rat antibody (pAb), HRP-coupled Goat Anti-Rat IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity) 2 mg
PK-AB718-9180 Rabbit Anti-Chicken antibody (pAb), AP-coupled Rabbit Anti-Chicken IgG conjugated to Alkaline Phosphatase (H&L) 1 mg
PK-AB718-9120 Rabbit Anti-Chicken antibody (pAb), HRP-coupled Rabbit Anti-Chicken IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity) 1.5 mg
PK-AB718-9380 Rabbit Anti-Goat antibody (pAb), AP-coupled Rabbit Anti-Goat IgG conjugated to Alkaline Phosphatase (H&L, minimum cross-reactivity) 0.5 mg
PK-AB718-9320 Rabbit Anti-Goat antibody (pAb), HRP-coupled Rabbit Anti-Goat IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity) 1 mg
PK-AB577-6403 Rabbit Anti-Goat IgG, HRP-coupled antibody (pAb) Rabbit Anti-Goat IgG conjugated to Horseradish Peroxidase 0.5 ml
PK-AB577-6402 Rabbit Anti-Mouse IgG, HRP-coupled antibody (pAb) Rabbit Anti-Mouse IgG conjugated to Horseradish Peroxidase 0.5 ml


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> Order Information PromoFluor Labeled Secondary Antibodies

 


Chemiluminescence Western Detection

PromoKine's ECL Chemiluminescence Detection Kit is a convenient, non-radioactive detection system containing all reagents required for highly sensitive chemiluminescence detection of immobilized proteins (Western blotting) or immobilized nucleic acids (Southern or Northern blotting) conjugated with HRP (Horse Radish Peroxidase) directly or indirectly. This ready-to-use kit allows to detect membrane immobilized specific antigens or nucleic acid sequences which are labeled either directly with HRP or indirectly with HRP-labeled antibodies/streptavidin.

Advantages

  • high sensitivity and resolution
  • stable hard copy results on film
  • only small amounts of antibody required
  • detection may be achieved in short exposure times (minutes)
  • excellent price/performance ratio
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Catalog Number Product Name Description Size Information Add to Cart
PK-MB902-500-1000 PromoKine ECL Chemiluminescence Detection Kit Kit for Chemiluminescent Detection of HRP or HRP-labeled Biomolecules 1000 ml (100 miniblots)
PK-MB902-500-120 PromoKine ECL Chemiluminescence Detection Kit Kit for Chemiluminescent Detection of HRP or HRP-labeled Biomolecules 120 ml (12 miniblots)
PK-MB902-500-500 PromoKine ECL Chemiluminescence Detection Kit Kit for Chemiluminescent Detection of HRP or HRP-labeled Biomolecules 500 ml (50 miniblots)
PK-MB902-889 PromoKine Hybridization Solution Hybridization Solution for Northern & Southern Blotting 100 ml
PK-MB902-805 PromoKine Super-Block Blocking Reagent for Western, Northern & Southern Blotting 10 g


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Antibody Labeling

PromKine provides a range of complete, ready-to-use Antibody Labeling Kits for conjugating antibodies to different fluorophores (e.g. PromoFluor dyes, phycobiliprotein dyes, fluorescein, rhodamine, sulforhodamine) as well as biotin, peroxidase and alkaline phosphatase.
Moreover, a variety of individual reactive dyes and phycobiliproteins for fluorescent labeling of antibodies is available as well.


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Note: Immunoblotting is also topic of a training course of our PromoCell Academy! >More Information