New Products & Highlights

Free Catalog

Get your free copy of the 2009/2010 PromoKine Cell Biology Catalog!

www.PromoCell.com

Have a look at our PromoCell website and find a broad range of primary human cells and media!

Secondary Detection

Labeled Antibodies

PromoKine offers high-quality secondary (polyclonal) antibodies at an excellent price / performance ratio. These antibodies are conjugated to horseradish peroxidase (HRP), Alkaline Phosphatase (AP), Biotin, Fluorescein, Texas Red or our superior PromoFluor fluorescent dyes. They can be used for different applications such as Western blotting, fluorescence microscopy and flow cytometry.

PromoFluor Labeled Secondary Antibodies

Catalog Number	Product Name	Description	Size
PK-AB718-9360	Donkey Anti-Goat antibody (pAb), Fluorescein-coupled	Donkey Anti-Goat IgG conjugated to Fluorescein (H&L)	2 mg
PK-AB718-9370	Donkey Anti-Goat antibody (pAb), Texas Red-coupled	Donkey Anti-Goat IgG conjugated to Texas Red (H&L)	2 mg
PK-AB718-9170	Goat Anti-Chicken antibody (pAb), Texas Red-coupled	Goat Anti-Chicken IgG conjugated to Texas Red (H&L)	2 mg
PK-AB718-9780	Goat Anti-Mouse antibody (pAb), AP-coupled	Goat Anti-Mouse IgG conjugated to Alkaline Phosphatase (H&L, minimum cross-reactivity)	1 mg
PK-AB718-9740	Goat Anti-Mouse antibody (pAb), Biotin-coupled	Goat Anti-Mouse IgG conjugated to Biotin (H&L, minimum cross-reactivity)	2 mg
PK-AB718-9760	Goat Anti-Mouse antibody (pAb), Fluorescein-coupled	Goat Anti-Mouse IgG conjugated to Fluorescein (H&L, minimum cross-reactivity)	2 mg
PK-AB718-9720	Goat Anti-Mouse antibody (pAb), HRP-coupled	Goat Anti-Mouse IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity)	2 mg
PK-AB718-9770	Goat Anti-Mouse antibody (pAb), Texas Red-coupled	Goat Anti-Mouse IgG conjugated to Texas Red (H&L)	2 mg
PK-AB718-9980	Goat Anti-Rabbit antibody (pAb), AP-coupled	Goat Anti-Rabbit IgG conjugated to Alkaline Phosphatase (H&L, minimum cross-reactivity)	1 mg
PK-AB718-9940	Goat Anti-Rabbit antibody (pAb), Biotin-coupled	Goat Anti-Rabbit IgG conjugated to Biotin (H&L, minimum cross-reactivity)	2 mg
PK-AB718-9960	Goat Anti-Rabbit antibody (pAb), Fluorescein-coupled	Goat Anti-Rabbit IgG conjugated to Fluorescein (H&L, minimum cross-reactivity)	2 mg
PK-AB718-9920	Goat Anti-Rabbit antibody (pAb), HRP-coupled	Goat Anti-Rabbit IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity)	2 mg
PK-AB718-9970	Goat Anti-Rabbit antibody (pAb), Texas Red-coupled	Goat Anti-Rabbit IgG conjugated to Texas Red (H&L)	2 mg
PK-AB577-6401	Goat Anti-Rabbit IgG, HRP-coupled antibody (pAb)	Goat Anti-Rabbit IgG conjugated to Horseradish Peroxidase	0.5 ml
PK-AB718-9580	Goat Anti-Rat antibody (pAb), AP-coupled	Goat Anti-Rat IgG conjugated to Alkaline Phosphatase (H&L, minimum cross-reactivity)	1 mg
PK-AB718-9520	Goat Anti-Rat antibody (pAb), HRP-coupled	Goat Anti-Rat IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity)	2 mg
PK-AB718-9180	Rabbit Anti-Chicken antibody (pAb), AP-coupled	Rabbit Anti-Chicken IgG conjugated to Alkaline Phosphatase (H&L)	1 mg
PK-AB718-9120	Rabbit Anti-Chicken antibody (pAb), HRP-coupled	Rabbit Anti-Chicken IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity)	1.5 mg
PK-AB718-9380	Rabbit Anti-Goat antibody (pAb), AP-coupled	Rabbit Anti-Goat IgG conjugated to Alkaline Phosphatase (H&L, minimum cross-reactivity)	0.5 mg
PK-AB718-9320	Rabbit Anti-Goat antibody (pAb), HRP-coupled	Rabbit Anti-Goat IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity)	1 mg
PK-AB577-6403	Rabbit Anti-Goat IgG, HRP-coupled antibody (pAb)	Rabbit Anti-Goat IgG conjugated to Horseradish Peroxidase	0.5 ml
PK-AB577-6402	Rabbit Anti-Mouse IgG, HRP-coupled antibody (pAb)	Rabbit Anti-Mouse IgG conjugated to Horseradish Peroxidase	0.5 ml

> Order Information PromoFluor Labeled Secondary Antibodies

Chemiluminescence Western Detection

PromoKine's ECL Chemiluminescence Detection Kit is a convenient, non-radioactive detection system containing all reagents required for highly sensitive chemiluminescence detection of immobilized proteins (Western blotting) or immobilized nucleic acids (Southern or Northern blotting) conjugated with HRP (Horse Radish Peroxidase) directly or indirectly. This ready-to-use kit allows to detect membrane immobilized specific antigens or nucleic acid sequences which are labeled either directly with HRP or indirectly with HRP-labeled antibodies/streptavidin.

Advantages

high sensitivity and resolution
stable hard copy results on film
only small amounts of antibody required
detection may be achieved in short exposure times (minutes)
excellent price/performance ratio

Catalog Number	Product Name	Description	Size
PK-MB902-500-1000	PromoKine ECL Chemiluminescence Detection Kit	Kit for Chemiluminescent Detection of HRP or HRP-labeled Biomolecules	1000 ml (100 miniblots)
PK-MB902-500-120	PromoKine ECL Chemiluminescence Detection Kit	Kit for Chemiluminescent Detection of HRP or HRP-labeled Biomolecules	120 ml (12 miniblots)
PK-MB902-500-500	PromoKine ECL Chemiluminescence Detection Kit	Kit for Chemiluminescent Detection of HRP or HRP-labeled Biomolecules	500 ml (50 miniblots)
PK-MB902-889	PromoKine Hybridization Solution	Hybridization Solution for Northern & Southern Blotting	100 ml
PK-MB902-805	PromoKine Super-Block	Blocking Reagent for Western, Northern & Southern Blotting	10 g

Antibody Labeling

PromKine provides a range of complete, ready-to-use Antibody Labeling Kits for conjugating antibodies to different fluorophores (e.g. PromoFluor dyes, phycobiliprotein dyes, fluorescein, rhodamine, sulforhodamine) as well as biotin, peroxidase and alkaline phosphatase.
Moreover, a variety of individual reactive dyes and phycobiliproteins for fluorescent labeling of antibodies is available as well.

Note: Immunoblotting is also topic of a training course of our PromoCell Academy! >More Information