pPK-CMV Reporter Vectors

• Suitable for in vitro and in vivo gene expression applications
• High plasmid yield in E. coli
• Expression ready with the following popular reporter genes: β-galactosidase, green fluorescent protein (GFP), luciferase, chloramphenicol acetyltransferase (CAT), secreted alkaline phosphatase (SEAP)
• Convenient multiple cloning site for optimized cloning and expression of target genes

The pPK-CMV Reporter Vectors have been designed for high level, transient expression of reporter or target genes. They contain an optimized CMV promoter followed by intron A from the CMV immediate-early gene (IE). This ensures extremely high transient expression in a wide variety of mammalian cells and tissues. In addition, the vectors contain an optimized plasmid backbone that has been extensively altered to achieve optimal plasmid production in E. coli.
The vectors are available without a reporter gene to clone and express a gene of interest (blank vector) as well as with the following reporter genes:
β-galactosidase (lacZ), green fluorescent protein (GFP), luciferase (Luc), chloramphenicol acetyltransferase (CAT), and secreted alkaline phosphatase (SEAP).

See also: Cell-based Reporter Assays

See also: Cell Transfection Reagents