pPK-CMV Expression Vectors

• High-level constitutive transgene expression
• Suitable for both stable (G418) and transient expression
• Expression of native and HA-tagged proteins
• Small size for increased transfection efficiency
• Convenient multiple cloning site containing the most commonly-used restriction sites

The pPK-CMV Expression Vectors contain a modified human cytomegalovirus (CMV) based promoter/enhancer sequence that has been optimized for high gene expression. Sequence motives that limit expression have been removed, while others that promote high expression evels have been retained. Consequently, these vectors yield significantly higher expression levels than other expression vectors such as pcDNA3.1 that are based on the standard CMV promoter.
In addition, the plasmid backbones are optimized to allow for very small vector sizes and higher plasmid yields when amplified in E. coli.
Three versions of the pPK-CMV Expression Vector are available:

pPK-CMV-E1 does not have an expression tag. It is intended for expressing recombinant proteins in the native forms.

pPK-CMV-E2 comes with an N-terminal HA (hemagglutinin) fusion tag.

pPK-CMV-E3 comes with a C-terminal HA-fusion tag. The HA fusion tags allow easy detection and purification of proteins.

All three Expression Vectors come with a positive control vector containing a CAT gene.

See also: Cell Transfection Reagents