Fluorescently Labeled Nucleotides

PromoKine is offering deoxynucleotides (dUTPs) labeled with our superior PromoFluor dyes. 

Aminoallyl-dUTP (5-aminoallyl-2'-deoxyuridine 5'-triphosphate) can be incorporated into DNA using conventional enzymatic incorporation techniques. PromoKine provides aminoallyl-dUTP conjugates with all of our premium PromoFluor dyes which cover the whole UV/VIS spectrum of interest. This selection of fluorescent labels is ideal for multicolor applications such as chromosome painting.

Compared to most other commercially available dye-labeled nucleotides they have excellent properties  with regard to signal intensity, water solubility, and stability. The lower molecular weight of the dyes results in minimal steric hindrance. Our fluorescently labeled aminoallyl-dUTPs are delivered as 1 mM ready-to-use aqueous solutions and can be used for different applications, e.g. fluorescent labeling of nucleic acids by PCR & RT-PCR, random primer and dTd labeling, in vitro transcription, mRNA FISH experiments, microarray applications, gene expression and binding studies, primer extension and nick translation assays.

  General PCR Labeling Protocol