Products

PromoFluor Reactive & Non-Reactive Dyes

All PromoFluor dyes are provided either as free carboxylic acids or in three reactive variants ready for covalent coupling to biomolecules:

  • Amine-reactive NHS-Esters
  • Thiol-reactive Maleimides
  • Amino-modified Labels


PromoFluor NHS-Esters

PromoFluor NHS-Esters (N-hydroxysuccinimidyl-esters) are intended for covalently labelling biomolecules containing a primary amino group (all peptides/proteins contain at least one amino group at the N-terminus). Moreover, amino groups can also be incorporated in other biomolecules without changing their activity or functionality. The most common examples are 5’-amino-modified DNA oligomers and cDNA containing aminoallyl-dU.

PromoFluor Maleimides

PromoFluor Maleimides are intended for covalently labeling biomolecules containing a thiol group (e.g. proteins). A neutral thiol-reactive label has little influence on the isoelectric point of a protein. In effect, PromoFluor Maleimides have an advantage over amino group labels because they do not interfere with techniques like differential 2D gel electrophoresis. Since most proteins contain only a few thiol functional groups, thiol-reactive dyes can be used to selectively modify a protein or peptide containing a thiol group at a specific site. Moreover, these dyes are well suited for labelling oligonucleotides. Maleimides couple to thiol groups under very mild conditions at a pH around 7.

PromoFluor Amino-Modified Labels

PromoFluor Amino-Modified Labels are intended for the covalent labeling of biomolecules containing a carboxy group e.g., glycoproteins. The amino group of PromoFluor Amino-Modified Labels can be cross-coupled to carboxy groups through a mild in situ activation.

Free Carboxylic Acids

The Carboxylic (Free) Acids are non-activated forms of our PromoFluor Dyes for use as controls and calibration factors in fluorescence imaging applications (e.g. for calculating background noise). These non-activated fluorescent dyes contain the native carboxyl group of the standard dye molecule.

Note: For labeling protocols please visit our Knowledge Base or contact our technical support.

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
PromoFluor-350, amino-modified label for coupling to e.g. carboxy groups 1 mg PK-PF350-2-01 click to select country
PromoFluor-350, carboxylic acid free carboxylic acid 5 mg PK-PF350-0-05 click to select country
PromoFluor-350, carboxylic acid free carboxylic acid 1 mg PK-PF350-0-01 click to select country
PromoFluor-350, maleimide for labeling thiol groups of proteins 5 mg PK-PF350-3-05 click to select country
PromoFluor-350, maleimide for labeling thiol groups of proteins 1 mg PK-PF350-3-01 click to select country
PromoFluor-350, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 mg PK-PF350-1-05 click to select country
PromoFluor-350, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 1 mg PK-PF350-1-01 click to select country
PromoFluor-350, NHS ester N-hydroxysuccinimidyl ester; For Labeling primary amino groups of proteins and amino-modified nucleic acid 5 x 0.2 mg PK-PF350-1-01A click to select country
PromoFluor-405, amino-modified label for coupling to e.g. carboxy groups 1 mg PK-PF405-2-01 click to select country
PromoFluor-405, carboxylic acid free carboxylic acid 5 mg PK-PF405-0-05 click to select country
PromoFluor-405, carboxylic acid free carboxylic acid 1 mg PK-PF405-0-01 click to select country
PromoFluor-405, maleimide for labeling thiol groups of proteins 5 mg PK-PF405-3-05 click to select country
PromoFluor-405, maleimide for labeling thiol groups of proteins 1 mg PK-PF405-3-01 click to select country
PromoFluor-405, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 mg PK-PF405-1-05 click to select country
PromoFluor-405, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 1 mg PK-PF405-1-01 click to select country
PromoFluor-405, NHS ester N-hydroxysuccinimidyl ester; For Labeling primary amino groups of proteins and amino-modified nucleic acid 5 x 0.2 mg PK-PF405-1-01A click to select country
PromoFluor-415, amino-modified label for coupling to e.g. carboxy groups 1 mg PK-PF415-2-01 click to select country
PromoFluor-415, carboxylic acid free carboxylic acid 5 mg PK-PF415-0-05 click to select country
PromoFluor-415, carboxylic acid free carboxylic acid 1 mg PK-PF415-0-01 click to select country
PromoFluor-415, maleimide for labeling thiol groups of proteins 5 mg PK-PF415-3-05 click to select country
PromoFluor-415, maleimide for labeling thiol groups of proteins 1 mg PK-PF415-3-01 click to select country
PromoFluor-415, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 mg PK-PF415-1-05 click to select country
PromoFluor-415, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 1 mg PK-PF415-1-01 click to select country
PromoFluor-415, NHS ester N-hydroxysuccinimidyl ester; For Labeling primary amino groups of proteins and amino-modified nucleic acid 5 x 0.2 mg PK-PF415-1-01A click to select country
PromoFluor-488 Premium, amino-modified label for coupling to e.g. carboxy groups 1 mg PK-PF488P-2-01 click to select country
PromoFluor-488 Premium, carboxylic acid free carboxylic acid 5 mg PK-PF488P-0-05 click to select country
PromoFluor-488 Premium, carboxylic acid free carboxylic acid 1 mg PK-PF488P-0-01 click to select country
PromoFluor-488 Premium, maleimide for labeling thiol groups of proteins 5 mg PK-PF488P-3-05 click to select country
PromoFluor-488 Premium, maleimide for labeling thiol groups of proteins 1 mg PK-PF488P-3-01 click to select country
PromoFluor-488 Premium, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 mg PK-PF488P-1-05 click to select country
PromoFluor-488 Premium, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 1 mg PK-PF488P-1-01 click to select country
PromoFluor-488 Premium, NHS ester N-hydroxysuccinimidyl ester; For Labeling primary amino groups of proteins and amino-modified nucleic acid 5 x 0.2 mg PK-PF488P-1-01A click to select country
PromoFluor-505, amino-modified label for coupling to e.g. carboxy groups 1 mg PK-PF505-2-01 click to select country
PromoFluor-505, maleimide for labeling thiol groups of proteins 5 mg PK-PF505-3-05 click to select country
PromoFluor-505, maleimide for labeling thiol groups of proteins 1 mg PK-PF505-3-01 click to select country
PromoFluor-505, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 mg PK-PF505-1-05 click to select country
PromoFluor-532, amino-modified label for coupling to e.g. carboxy groups 1 mg PK-PF532-2-01 click to select country
PromoFluor-532, carboxylic acid free carboxylic acid 5 mg PK-PF532-0-05 click to select country
PromoFluor-532, carboxylic acid free carboxylic acid 1 mg PK-PF532-0-01 click to select country
PromoFluor-532, maleimide for labeling thiol groups of proteins 5 mg PK-PF532-3-05 click to select country
PromoFluor-532, maleimide for labeling thiol groups of proteins 1 mg PK-PF532-3-01 click to select country
PromoFluor-532, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 mg PK-PF532-1-05 click to select country
PromoFluor-532, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 1 mg PK-PF532-1-01 click to select country
PromoFluor-532, NHS ester N-hydroxysuccinimidyl ester; For Labeling primary amino groups of proteins and amino-modified nucleic acid 5 x 0.2 mg PK-PF532-1-01A click to select country
PromoFluor-546, amino-modified label for coupling to e.g. carboxy groups 1 mg PK-PF546-2-01 click to select country
PromoFluor-546, carboxylic acid free carboxylic acid 5 mg PK-PF546-0-05 click to select country
PromoFluor-546, carboxylic acid free carboxylic acid 1 mg PK-PF546-0-01 click to select country
PromoFluor-546, maleimide for labeling thiol groups of proteins 5 mg PK-PF546-3-05 click to select country
PromoFluor-546, maleimide for labeling thiol groups of proteins 1 mg PK-PF546-3-01 click to select country
PromoFluor-546, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 mg PK-PF546-1-05 click to select country
PromoFluor-546, NHS ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 1 mg PK-PF546-1-01 click to select country
PromoFluor-546, NHS ester N-hydroxysuccinimidyl ester; For Labeling primary amino groups of proteins and amino-modified nucleic acid 5 x 0.2 mg PK-PF546-1-01A click to select country
PromoFluor-555P, amino-modified label For coupling to e.g. carboxy groups 1 mg PK-PF555P-2-01 click to select country
PromoFluor-555P, carboxylic acid Free carboxylic acid 1 mg PK-PF555P-0-01 click to select country
PromoFluor-555P, carboxylic acid Free carboxylic acid 5 mg PK-PF555P-0-05 click to select country
PromoFluor-555P, maleimide For labeling thiol groups of proteins 1 mg PK-PF555P-3-01 click to select country
PromoFluor-555P, maleimide For labeling thiol groups of proteins 5 mg PK-PF555P-3-05 click to select country
PromoFluor-555P, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 1 mg PK-PF555P-1-01 click to select country
PromoFluor-555P, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 x 0.2 mg PK-PF555P-1-01A click to select country
PromoFluor-555P, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 mg PK-PF555P-1-05 click to select country
PromoFluor-568, amino-modified label For coupling to e.g. carboxy groups 1 mg PK-PF568-2-01 click to select country
PromoFluor-568, carboxylic acid Free carboxylic acid 1 mg PK-PF568-0-01 click to select country
PromoFluor-568, carboxylic acid Free carboxylic acid 5 mg PK-PF568-0-05 click to select country
PromoFluor-568, maleimide For labeling thiol groups of proteins 1 mg PK-PF568-3-01 click to select country
PromoFluor-568, maleimide For labeling thiol groups of proteins 5 mg PK-PF568-3-05 click to select country
PromoFluor-568, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 1 mg PK-PF568-1-01 click to select country
PromoFluor-568, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 x 0.2 mg PK-PF568-1-01A click to select country
PromoFluor-568, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 mg PK-PF568-1-05 click to select country
PromoFluor-594, amino-modified label For coupling to e.g. carboxy groups 1 mg PK-PF594-2-01 click to select country
PromoFluor-594, carboxylic acid Free carboxylic acid 1 mg PK-PF594-0-01 click to select country
PromoFluor-594, carboxylic acid Free carboxylic acid 5 mg PK-PF594-0-05 click to select country
PromoFluor-594, maleimide For labeling thiol groups of proteins 1 mg PK-PF594-3-01 click to select country
PromoFluor-594, maleimide For labeling thiol groups of proteins 5 mg PK-PF594-3-05 click to select country
PromoFluor-594, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 1 mg PK-PF594-1-01 click to select country
PromoFluor-594, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 x 0.2 mg PK-PF594-1-01A click to select country
PromoFluor-594, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 mg PK-PF594-1-05 click to select country
PromoFluor-633P, amino-modified label For coupling to e.g. carboxy groups 1 mg PK-PF633P-2-01 click to select country
PromoFluor-633P, carboxylic acid Free carboxylic acid 1 mg PK-PF633P-0-01 click to select country
PromoFluor-633P, carboxylic acid Free carboxylic acid 5 mg PK-PF633P-0-05 click to select country
PromoFluor-633P, maleimide For labeling thiol groups of proteins 1 mg PK-PF633P-3-01 click to select country
PromoFluor-633P, maleimide For labeling thiol groups of proteins 5 mg PK-PF633P-3-05 click to select country
PromoFluor-633P, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 1 mg PK-PF633P-1-01 click to select country
PromoFluor-633P, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 x 0.2 mg PK-PF633P-1-01A click to select country
PromoFluor-633P, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 mg PK-PF633P-1-05 click to select country
PromoFluor-647P, amino-modified label For coupling to e.g. carboxy groups 1 mg PK-PF647P-2-01 click to select country
PromoFluor-647P, carboxylic acid Free carboxylic acid 1 mg PK-PF647P-0-01 click to select country
PromoFluor-647P, carboxylic acid Free carboxylic acid 5 mg PK-PF647P-0-05 click to select country
PromoFluor-647P, maleimide For labeling thiol groups of proteins 1 mg PK-PF647P-3-01 click to select country
PromoFluor-647P, maleimide For labeling thiol groups of proteins 5 mg PK-PF647P-3-05 click to select country
PromoFluor-647P, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 1 mg PK-PF647P-1-01 click to select country
PromoFluor-647P, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 x 0.2 mg PK-PF647P-1-01A click to select country
PromoFluor-647P, NHS-Ester N-hydroxysuccinimidyl ester; for labeling primary amino groups of proteins and amino-modified nucleic acid 5 mg PK-PF647P-1-05 click to select country