Fluorescent labeling with PromoFluor dyes. Illustrations (except illustration at the top) are from Covalys Biosciences AG/Switzerland.

PromoFluor Fluorescent Dyes

For sensitive detection of biomolecules and their interactions and studying the manifold cellular processes in a more convenient way, PromoKine now provides a wide range of powerful fluorophores spanning almost the whole visible and near infrared light spectrum.

The superior quality of our PromoFluor dyes and a very reasonable price making them an ideal alternative to many commonly used fluorophores such as the Alexa Fluor® or Cy™ dyes. They are compatible with most excitation sources including low cost diode lasers, LEDs, tungsten lamps and xenon arc lamps and can be excited with custom made FRET systems.

The activated PromoFluor dyes are ready to use and can be covalently coupled to amino acids, proteins, antibodies, hormones, sugars, dNTPs, oligonucleotides or other affinity tags (e.g. biotin or streptavidin) to obtain the required specific probes or markers for highly sensitive cell and molecular biological applications such as immunofluorescence microscopy, flow cytometry, studies of enzyme kinetics, DNA sequencing, FISH, and microarray assays (some may also be suitable for FRET assays).

In addition, we are also offering dyes with an extra large Stokes shift (PromoFluor LSS dyes), which are particularly useful e.g. for multicolor labeling as well as a selection of near infrared (NIR) dyes.

Note: For labeling protocols please visit our Knowledge Base or contact our technical support.

Selection of Currently Available PromoFluor Dyes

PromoFluor label absorption maximum [nm] emission maximum [nm] excellent alternative to: laser excitation source
PF-350 354 431 Alexa Fluor 350, AMCA, AMCA-X Argon laser; UV-light
PF-405 400 420 Alexa Fluor 405, Pacific Blue, Cascade Blue Blue diode laser; Violet 405 krypton laser
PF-415 418 467 Pacific Blue, Cascade Blue Blue diode laser; Violet 405 krypton laser
PF-488 Premium 490 516 Fluorescein (FITC), Alexa Fluor 488, Cy2 Argon 488 blue-green laser
PF-505 505 530 RhodamineGreen Argon 488 blue-green laser
PF-532 539 561 Alexa Fluor 532, Tetramethylrhodamine (TMR, TRITC) Nd:YAG laser
PF-546 551 572 Alexa Fluor 546, Tetramethylrhodamine (TMR) Helium-Neon 543 green laser
PF-555P 557 571 Alexa Fluor 555, Cy3, Tetramethylrhodamine (TMR) Helium-Neon 543 green laser
PF-568 580 598 TexasRed, Alexa Fluor 568, Cy3.5, Rhodamine Red/Rhodamine B dye Helium-Neon 594 laser; Ar-Kr mixed-gas laser
PF-594 594 615 TexasRed, Alexa Fluor 594 Helium-Neon 594 laser
PF-633P 635 658 Alexa Fluor 633 Helium-Neon 633 laser; Red diode 635 laser
PF-647P 654 672 Alexa Fluor 647, Cy5, Allophycocyanin Krypton-Argon 647 laser
PF-670 673 694 Alexa Fluor 660, Alexa Fluor 680, Cy5.5 Helium-Neon laser
PF-680 690 709 Alexa Fluor 680, Cy5.5 Helium-Neon laser
PF-700P 706 731 Alexa Fluor 700 Far-red diode lasers; xenon-arc lamp; dye-pumped laser
PF-750 748 772 Alexa Fluor 750, Cy7 Far-red diode lasers; xenon-arc lamp; dye-pumped lasers
PF-770 769 796 Alexa Fluor 750, Cy7 xenon-arc lamp; dye-pumped laser
PF-780 783 800 NIR dye xenon-arc lamp; dye-pumped laser
PF-840 844 884 NIR dye xenon-arc lamp; dye-pumped laser
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Looking for better alternatives....

PromoFluor-350 is an intense, blue-fluorescing dye that is ideally suited for UV light excitation. With an emission maximum at 431 nm (that is shorter than that of e.g. AMCA), its bright blue fluorescence can be better separated from that of common green fluorescence dyes.

PromoFluor-405 is an optimal visible light-excitable blue fluorophore and ideal for 400-410 nm excitation sources (e.g. the 405 nm line of the krypton laser). Since it has minimal spectral overlap with green fluorophores, it is well-suited for multicolor labeling.

PromoFluor-488P (Premium) is the perfect choice if you want to replace green emitting fluorophores such as Cy™2, Alexa Fluor® 488 and fluorescein. They are brighter than fluorescein and show a better water solubility and pH insensitivity over a broad range. In addition, they are significantly more photostable than fluorescein - particularly PromoFluor-488 Premium that is thus ideally suited for applications such as fluorescence microscopy. The dyes are compatible to the Argon 488 blue-green laser. Exc./Em. maximum: 490/516 nm, respectively.

PromoFluor-555P is the ideal alternative to TMR as well as the red fluorescent Cy™3 and Alexa Fluor® 555 dyes. Biomolecules (antibodies, nucleic acids) labeled with this dye are well compatible to TMR and Cy™3 filters. Exc./Em. maximum: 557/571 nm.

PromoFluor-594 is a good alternative to TexasRed® and Alexa Fluor 594 emitting bright red fluorescence. Biomolecules labeled with this dye are well compatible to Texas Red® filters. Exc./Em. maximum: 594/615 nm.

PromoFluor-633P is optimally suited to be excited with the 633 nm spectral line of the He-Ne laser and the 635 nm line of red diode lasers and can be used instead of Alexa Fluor® 633. The bright and photostable dye emits highly intensive red fluorescence. Exc./Em. maximum: 635/658 nm.

PromoFluor-647P is an ideal replacement of Cy™5, Alexa Fluor® 647 and allophycocyanin producing intense red fluorescence emission. It is very photostable and can be optimally excited with the 647 nm spectral line of the Krypton-Argon laser. Exc./Em. maximum: 654/672 nm.

PromoFluor-680 is optimal if you are looking for an alternative to Cy™5.5 or Alexa Fluor® 680 and emits intense fluorescence in the far-infrared region of the light spectrum. Ex./Em. maximum: 690/709 nm.

PromoFluor-750 is an near-infrared (NIR) dye with a spectrum similar to Cy™7. Its fluorescence emission with a maximum at 776 nm can be well separated from commonly used red fluorophores such as PromoFluor-647 or Cy™5. With an excitation peak at 747 nm, conjugates of PromoFluor-750 can be excited well by a xenon arc lamp, far-red diode lasers or dye-pumped lasers.

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