Products

Animal-free, endotoxin-free Cytokines & Growth Factors

PromoKine's animal-free cytokines and growth factors have been produced by molecular farming using barley grain or tobacco plants as production host - which has also the G.R.A.S. (Generally Recognized As Safe) status from the FDA.
These cytokines are free of animal components, serum, endotoxins and antibiotics as well as human or animal infectious agents or other endogenous mammalian contamination (e.g. traces of unwanted growth factors and cytokines). As these proteins have been expressed in barley seed endosperm or tobacco plants, they show very low proteolytic, pyrogenic and inflammatory activity compared to other commercially available proteins produced in bacteria, yeast, insect or mammalian cells. In addition, they retain proper eukaryotic folding and glycosylation resulting in an increased stability and biological activity. Degradation due to protease carryover as well as unwanted cell responses (e.g. proliferation, chemokine release and inflammatory cascades) or cell growth inhibition caused by contaminating serum components (serum carryover), endotoxins or bacterial, viral and prion infectious agents are eliminated or at least extremely reduced.

Note: Endotoxins (lipopolysaccharides, LPS) are produced and released by Gram-negative bacteria. They are frequently contaminating protein preparations derived e.g. from E. coli and are difficult to remove. Cell culture experiments have shown that even low levels of endotoxins currently regarded as acceptable (~1 EU/µg) and found in many commercially available cytokines and growth factors can have profound effects on cell viability as well as cellular behaviour and functions. In particular, even low endotoxin traces found in many cytokines and growth factors produced in E. coli (indicating endotoxin contents <1 EU/µg) may be quite critical for stem cell experiments as final endotoxin concentrations of 0.001 ng/ml in the medium can already have significant effects on stem cell cultivation (e.g. failure to differentiate into functional cell types, general adverse effects on cell viability and proliferation). Endotoxin traces in E. coli-derived cytokines and growth factors are also biasing angiogenesis studies as they show considerable angiogenic effects inducing tumour cell proliferation and VEGF production and decreasing apoptosis.

PromoKine's animal-free cytokines and growth factors typically have an endotoxin level of less than 0.05 EU/µg (i.e. they are virtually free of endotoxins) excluding such complications in cell culture.

Note: We now also provide endotoxin-free HSA that can be used as carrier protein for long-term storage of cytokines and growth factors in solution.

 

 Test it and request your FIRST ORDER DISCOUNT

 

 


 

 

References, Questions & Answers

B

back to top
Product Name Description Size Catalog Number Information Prices
BMP-2, human, recombinant (Plant) Recombinant Human Bone Morphogenetic Protein 2 (plant-derived) 10 µg C-67310A click to select country

E

back to top
Product Name Description Size Catalog Number Information Prices
EGF, human, recombinant (Plant) Recombinant Human Epidermal Growth Factor (plant-derived) 100 µg C-60169A click to select country

F

back to top
Product Name Description Size Catalog Number Information Prices
FGF-2 (FGF-b, 146 aa), human, recombinant (Plant) Recombinant Human basic Fibroblast Growth Factor 2 (FGF-b, 146 amino acid variant; plant-derived) 50 µg C-60242A click to select country
flt-3 Ligand, human, recombinant (Plant) Recombinant Human flt-3 Ligand (plant-derived) 10 µg C-67110A click to select country

G

back to top
Product Name Description Size Catalog Number Information Prices
G-CSF, human, recombinant (Plant) Recombinant Human Granulocyte Colony Stimulating Factor (plant-derived) 10 µg C-60434A click to select country
GDNF, human, recombinant (Plant) Recombinant Human Glial Derived Neurotropic Factor (plant-derived) 10 µg C-66312A click to select country
GM-CSF, human, recombinant (Plant) Recombinant Human Granulocyte/Macrophage Colony Stimulating Factor (plant-derived) 10 µg C-60420A click to select country

H

back to top
Product Name Description Size Catalog Number Information Prices
HSA, human, recombinant (Plant) Recombinant Human Serum Albumin (plant-derived) 10 mg C-69500A click to select country

I

back to top
Product Name Description Size Catalog Number Information Prices
IFN-gamma, human, recombinant (Plant) Recombinant Human Interferon gamma (plant-derived) 100 µg C-60724A click to select country
IL-2, human, recombinant (Plant) Recombinant Human Interleukin 2 (plant-derived) 50 µg C-61240A click to select country
IL-3, human, recombinant (Plant) Recombinant Human Interleukin 3 (plant-derived) 10 µg C-61330A click to select country
IL-4, human, recombinant (Plant) Recombinant Human Interleukin 4 (plant-derived) 10 µg C-61420A click to select country

K

back to top
Product Name Description Size Catalog Number Information Prices
KGF (FGF-7), human, recombinant (Plant) Recombinant Human Keratinocyte Growth Factor (FGF-7; plant-derived) 10 µg C-63820A click to select country

L

back to top
Product Name Description Size Catalog Number Information Prices
LIF, human, recombinant (Plant) Recombinant Human Leukemia Inhibitory Factor (plant-derived) 50 µg C-60051A click to select country
LIF, mouse, recombinant (Plant) Recombinant Mouse Leukemia Inhibitory Factor (plant-derived) 50 µg D-60051A click to select country

M

back to top
Product Name Description Size Catalog Number Information Prices
M-CSF, human, recombinant (Plant) Recombinant Human Macrophage Colony Stimulating Factor (plant-derived) 10 µg C-60442A click to select country

S

back to top
Product Name Description Size Catalog Number Information Prices
SCF, human, recombinant (Plant) Recombinant Human Stem Cell Factor (plant-derived) 10 µg C-63120A click to select country
SCF, mouse, recombinant (Plant) Recombinant Mouse Stem Cell Factor (plant-derived) 10 µg D-63120A click to select country

T

back to top
Product Name Description Size Catalog Number Information Prices
TNF-alpha, human, recombinant (Plant) Recombinant Human Tumor Necrosis Factor alpha (plant-derived) 50 µg C-63720A click to select country

V

back to top
Product Name Description Size Catalog Number Information Prices
VEGF-165, human, recombinant (Plant) Recombinant Human Vascular Endothelial Cell Growth Factor 165 (plant-derived) 10 µg C-64420A click to select country


back to top