• superior transfection efficiencies with many cell types
• lowest cytotoxicity with most cell lines - gentle to sensitive cells
• suited for stable and transient transfections
• broad cell-line spectrum - ideally suited also for primary cell transfection
• simple, straightforward and time-saving protocol
• compatible with serum and antibiotics
• remarkably reproducible results
• cell-specific versions for endothelial cells (e.g. HUVEC or HDMEC), macrophages and hepatocytes available

Applications

• efficient transfection of various mammalian cell types - including primary cells
• transient and stable transfections
• suited for delivery of plasmid DNA, oligonucleotides and siRNA
• optimized protocols for HTS applications and protein production available

PromoFectin provides highly effective and reliable delivery of nucleic acids into a variety of cell types, including many hard-to-transfect cell lines and primary cells such as human endothelial cells, fibroblasts and hepatocytes. It consists of a proprietary non-liposomal, cationic polymer that is stable, water-soluble and free of any components of animal origin. In contrast to many liposome-based transfection reagents, it shows very low toxicity and is therefore ideally suited even for transfection of very sensitive cells yielding optimal transfection efficiencies and gene expression. Transfection with PromoFectin typically requires no medium change and it can be used for highly effective delivery of plasmid DNA as well as oligonucleotides and siRNA into adherent and non-adherent cells. It compacts DNA into positively-charged, stable and protective complexes which bind to negatively-loaded cell-surface residues and enter the cell by endocytosis. In the endosome, PromoFectin acts as a "proton sponge", buffering the endosomal pH that leads to pH inhibition of endosomal nucleases and ensures stability of the complexes. Continuous proton influx leads to osmotic swelling and rupture of the endosome, allowing a very rapid and almost complete release of the nucleic acids into the cytosol. Moreover, PromoFectin also favors DNA trafficking to the cell nucleus - a property that is particularly useful for transfection of slow-dividing or resting cells.

Please request an offer for testing PromoFectin!

PromoFectin-Macrophage has been developed for optimal transfection of the hard-to-transfect macrophages (e.g. primary human macrophages derived from blood monocytes) and macrophage-derived cell lines such as RAW 264.7 and works also with monocytes and dendritic cells. PromoFectin-Macrophage is a mannose-bearing variant of our standard PromoFectin reagent that binds to cells expressing mannose-specific membrane receptors - such as macrophages, monocytes and dendritic cells - leading to enhanced internalization of the DNA complexes. PromoFectin-Macrophage is compatible with serum and antibiotics, thus eliminating the need for media changes.  

Please request an offer for testing PromoFectin-Macrophage!