Products

MATra Protocols & Specifications

Easy Protocol

Example with MATra-A Reagent

  1. Dilute nucleic acid in medium

  2. Add magnetic nanoparticle (MATra-A Reagent)

  3. Incubate 20 - 30 minutes

  4. Add medium to adherent cells (2 - 4 x 105 cells)

  5. Add nucleic acid/nanoparticle solution

  6. Place culture plate on magnet plate

  7. Incubate 15 minutes

  8. Remove magnet plate

  9. Make medium change (recommended for sensitive cell types - e.g. primary cells)

Note: the loading of MATra-A Reagent with nucleic acids has to be performed under serum-free conditions while the nucleic acids loaded MATra-A magnetic particles can be applied to the cells in the presence of serum

MATra Specifications

MATra is applicable at different phases of cell confluence, however in general we recommend 60 - 80% confluence (in some systems a higher visual confluence may result in higher Magnet Assisted Transfection rates).

MATra is non-toxic at recommended amounts in most cell systems. However, if higher MATra/nucleic acid amounts are used, a medium change is recommended after 1 - 2 h. For transfection of very sensitive cells (e.g. some primary cells), we recommend medium change directly after the transfection procedure and adjusting the amount of the MATra-A/nucleic acid concentration individually. Reducing the MATra-A concentration might result in better cell viability without decreasing transfection efficiency significantly.

 

The MATra technology is compatible with robots for high-throughput transfections!

 

Low Vector Doses

Magnet Assisted Transfection and lipofection in comparison. Luciferase expression was assayed after 24 hours.

Assay Formats

Formats Volume of MATra-A reagent recommended [µl] Transfections per vial (200 µl vial)*
96 well plate 0.1 2000
48 well plate 0.3 667
24 well plate 0.6 333
12 well plate 1.2 167
6 well plate 3 67
60 mm dish 6.6 30
100 mm dish 17.2 12
T-75 flask 23.5 9
26x26 cm plate 156 1**