MATra - Protocols & Specifications
MATra Products
- We recommend using both procedures in parallel to determine the optimal method for your particular cells of interest: Magnet Assisted Transfection using MATra-A Reagent and Magnet Assisted Lipofection using MA Lipofection Enhancer with a standard transfection reagent of choice.
Easy Protocol
Example with MATra-A Reagent
1. Dilute nucleic acid in medium
2. Add magnetic nanoparticle (MATra-A Reagent)
3. Incubate 20 - 30 minutes
4. Add medium to adherent cells (2 - 4 x 105 cells)
5. Add nucleic acid/nanoparticle solution
6. Place culture plate on magnet plate
7. Incubate 15 minutes
8. Remove magnet plate
9. Make medium change (recommended for sensitive cell types - e.g. primary cells)
Note: the loading of MATra-A Reagent with nucleic acids has to be performed under serum-free conditions while the nucleic acids loaded MATra-A magnetic particles can be applied to the cells in the presence of serum
MATra Specifications
MATra is applicable at different phases of cell confluence, however in general we recommend 60 - 80% confluence (in some systems a higher visual confluence may result in higher Magnet Assisted Transfection rates).
MATra is non-toxic at recommended amounts in most cell systems. However, if higher MATra/nucleic acid amounts are used, a medium change is recommended after 1 - 2 h. For transfection of very sensitive cells (e.g. some primary cells), we recommend medium change directly after the transfection procedure and adjusting the amount of the MATra-A/nucleic acid concentration individually. Reducing the MATra-A concentration might result in better cell viability without decreasing transfection efficiency significantly.
The MATra technology is compatible with robots for high-throughput transfections!
Low Vector Doses
- Magnet Assisted Transfection and lipofection in comparison. Luciferase expression was assayed after 24 hours.
Assay Formats
| Formats | Volume of MATra-A reagent recommended [µl] | Transfections per vial (200 µl vial)* |
| 96 well plate | 0.1 | 2000 |
| 48 well plate | 0.3 | 667 |
| 24 well plate | 0.6 | 333 |
| 12 well plate | 1.2 | 167 |
| 6 well plate | 3 | 67 |
| 60 mm dish | 6.6 | 30 |
| 100 mm dish | 17.2 | 12 |
| T-75 flask | 23.5 | 9 |
| 26x26 cm plate | 156 | 1** |
Note: Cell Transfection (e.g. transfection of primary human cells) is also topic of a training course of our PromoCell Academy! >More Information