Products

Senescence Detection Kits

Senescence is an arrested state in which the cells remain viable, but are not stimulated to divide by serum or passage in culture. It is thought to be a tumor-suppressive mechanism and an underlying cause of aging. Senescent cells display an increase of cell size, senescence-associated expression of β-galactosidase (SAβ-Gal) activity, and altered patterns of gene expression.

The PromoKine Senescence Detection Kits have been designed to detect this specific senescence marker (SA-ß-Gal activity) either histochemically in cultured cells and tissue sections (Senescence Detection Kit I) or fluorometrically in adherent and suspension cells by a simple one-step protocol using flow cytometry (Senescence Detection Kit II). The specific marker is only present in senescent cells and is not found in presenescent, quiescent, or immortal cells.

Cell Cycle Analysis Kit

PromoKine’s Cell Cycle Analysis Kit provides a quick and easy method to detect the number of cells, which are at a specific stage of the cell cycle. The kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in a fluorescent signal, which is proportional to cellular DNA content. The percentages of cells in different phases of the cell cycle (G0/G1, S, and G2/M) can be quantified by flow cytometry.

The method is non-radioactive, rapid and accurate and can be used for high-throughput cell cycle analysis with contemporary flow cytometer instruments.

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
Cell Cycle Analysis Kit Flow cytometry-based method to detect and monitor cells at various stages of the cell cycle. 100 assays PK-CA577-K920 click to select country
Senescence Detection Kit I (histochemical) Specific detection of senescence marker in distinct pH in cultured cells and tissue sections 250 assays PK-CA577-K320 click to select country
Senescence Detection Kit II (fluorometric) Simple, sensitive, one-step assay to measure senescence cells using FACS. 100 assays PK-CA577-K991 click to select country
SKPin C1 2-[4-Bromo-2-[[4-oxo-3-(3-pyridinyl­methyl)-2-thioxo-5-thiazolidinylidene]methyl]pheno­xy]acetic acid; promotes cell type-specific blocking of cell cycle in G1 or G2/M phase. 5 mg PK-CA577-2343-5 click to select country
SKPin C1 2-[4-Bromo-2-[[4-oxo-3-(3-pyridinyl­methyl)-2-thioxo-5-thiazolidinylidene]methyl]pheno­xy]acetic acid; promotes cell type-specific blocking of cell cycle in G1 or G2/M phase. 25 mg PK-CA577-2343-25 click to select country
VK3-OCH3 2-[(2-Methoxyethyl)thio]-3-methyl-1,4-naphthalenedione; VK3-Ome; A potent antitumor agent. Induces G2/M cell cycle arrest and apoptosis. 25 mg PK-CA577-2694-25 click to select country
VK3-OCH3 2-[(2-Methoxyethyl)thio]-3-methyl-1,4-naphthalenedione; VK3-Ome; A potent antitumor agent. 2-[(2-Methoxyethyl)thio]-3-methyl-1,4-naphthalenedione; VK3-Ome; A potent antitumor agent. Induces G2/M cell cycle arrest and apoptosis. 5 mg PK-CA577-2694-5 click to select country