Non-fluorescent DNA Labeling
For convenient biotin-labeling of DNA by PCR
PromoKine's Biotin PCR Labeling Kit contains all reagents required for convenient PCR labeling of DNA with biotin (except primer and template) providing a highly efficient, easy-to-perform and rapid labeling technology. In combination with streptavidin/avidin-conjugated fluorophores (e.g. PromoFluor or phycobiliproteins) or enzymes (e.g. horseradish peroxidase), Biotin-labeled DNA can be used for many applications such as Southern and Northern blotting, dot blots, DNA microarrays, immobilization of DNA.
The biotin-labeled dNTPs are suited for direct enzymatic labeling of DNA and are optimized for incorporation into DNA/cDNA by a variety of enzymes. Recommended labeling methods include PCR with Taq Polymerase, Nick Translation with DNAse I/ DNA Polymerase, Primer Extension with Klenow exo-, Reverse Transcription with MMLV Reverse Transcriptase, and 3'-end labeling with Terminal deoxynucleotidyl Transferase (TdT).
References, Questions & Answers
see also: Fluorescent labeling of DNA