Products

Non-fluorescent DNA Labeling

For convenient biotin-labeling of DNA by PCR

PromoKine's Biotin PCR Labeling Kit contains all reagents required for convenient PCR labeling of DNA with biotin (except primer and template) providing a highly efficient, easy-to-perform and rapid labeling technology. In combination with streptavidin/avidin-conjugated fluorophores (e.g. PromoFluor or phycobiliproteins) or enzymes (e.g. horseradish peroxidase), Biotin-labeled DNA can be used for many applications such as Southern and Northern blotting, dot blots, DNA microarrays, immobilization of DNA. 

The biotin-labeled dNTPs are suited for direct enzymatic labeling of DNA and are optimized for incorporation into DNA/cDNA by a variety of enzymes. Recommended labeling methods include PCR with Taq Polymerase, Nick Translation with DNAse I/ DNA Polymerase, Primer Extension with Klenow exo-, Reverse Transcription with MMLV Reverse Transcriptase, and 3'-end labeling with Terminal deoxynucleotidyl Transferase (TdT).

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
Biotin PCR Labeling Kit Kit for biotin-labeling of DNA by PCR 500 reactions PK-CA-BIO-LK500 click to select country
Biotin PCR Labeling Kit Kit for biotin-labeling of DNA by PCR 100 reactions PK-CA-BIO-LK100 click to select country
Biotin-dATP Biotin-conjugated propargylamino-dATP (1 mM) 50 µl PK-CA-BIO-DATP-50P click to select country
Biotin-dATP Biotin-conjugated propargylamino-dATP (1 mM) 10 µl PK-CA-BIO-DATP-10P click to select country
Biotin-dCTP Biotin-conjugated propagylamino-dCTP (1 mM) 200 µl PK-CA-BIO-DCTP-200P click to select country
Biotin-dCTP Biotin-conjugated propagylamino-dCTP (1 mM) 1 ml PK-CA-BIO-DCTP-1000P click to select country
Biotin-dUTP Biotin-conjugated aminoallyl-dUTP (1 mM) 1 ml PK-CA-BIO-DUTP-1000P click to select country
Biotin-dUTP Biotin-conjugated aminoallyl-dUTP (1 mM) 200 µl PK-CA-BIO-DUTP-200P click to select country


see also: Fluorescent labeling of DNA