Applications

• DNA/RNA isolation
• Various molecular genetic applications

Benefits

• Fast, simple and reproducible procedures
• Optimal yields
• Highly pure nucleic acids suitable for many applications
• Reduced toxicity due to replacement of hazardous compounds

PromoKine DNA Isolation Kit

This easy-to-use DNA Isolation Kit can be used for efficiently extracting of DNA from agarose gels, isolating plasmid DNA from mini-preps, and for concentrating DNA without ethanol precipitation. The entire purification procedures are quick and yield purest DNA that can be used in ligations, PCR, cloning, probe construction, sequencing, e

Genomic DNA Isolation Kit I

The Genomic DNA Isolation Kit I provides a simple and convenient procedure for rapid isolation of genomic DNA from mammalian cells and tissue samples with high yield and purity. The novel method requires less than 90 minutes to prepare highly pure genomic DNA that is free from protein and RNA and is suitable for a variety of applications such as PCR, DNA hybridization, cloning, Southern blot, and array-based experiments.

Genomic DNA Isolation Kit II

The Genomic DNA Isolation Kit II contains a non-organic and ready-to-use reagent for the isolation of genomic DNA from samples of human, animal, plant, yeast, bacterial, and viral origin. There is no phenol step included. The procedure is fast and can be completed in 10-30 minutes with DNA recovery of 70-100% - permitting efficient isolation of genomic DNA from a large number of samples of small or large volumes. The isolated DNA can be used, without additional purification, for Southern analysis, dot blot hybridization, molecular cloning, RFLP, PCR, and other molecular biology applications.

The separately available Plant DNA Isolation Buffer binds the reactive polyphenolic and polysaccharide contaminants present in plant tissues. The also separately available  Blood DNA Isolation Buffer is intendedhas been developed for the isolation of genomic DNA from frozen or fresh blood. Both isolation buffers are intended for use with the PromoKine Genomic DNA Isolation Kit II.

Mitochondrial DNA Extraction Kit

The Mitochondrial DNA Extraction Kit provides a convenient tool for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications.

PCR Purification Kit

The PromoKine PCR Purification Kit has been designed for the work-up of PCR reactions (removal of primer dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities). The preparation is based on a silica-membrane technology for binding DNA in high-salt and elution in low-salt buffer. The kit provides a simple and efficient way to purify linear or circular DNA and is optimized for working with DNA amounts of up to 50 µg. It requires no organic extractions or precipitation and guarantees high and stable recovery rates.

Total RNA Isolation Kit I

The Total RNA Isolation Kit I is a complete kits with ready-to-use reagents for the isolation of total RNA from samples of human, animal, plant, yeast, bacterial, or viral origin. It is based on disrupting cells in guanidine thiocyanate/detergent solution, followed by organic extraction and alcohol precipitation of the RNA, and the kit allows simultaneous processing of a large number of sample

Total RNA Isolation Kit II

In the Total RNA Isolation Kit II, chloroform is replaced by 1-Bromo-3-chloropropane (BCP) - thus reducing toxic material handling without any reverse effects on the quality of isolated RNA, DNA, or proteins.

The resulting RNA is suitable for the isolation of Poly A⁺ RNA or for Northern blotting, dot blotting, in vitro translation, cloning, RT-PCR, RNase protection assays, or other analytical procedures.

The RBC Lysis Solution selectively lyses erythrocytes and is intended for use in the isolation of leukocytes from whole blood. It can be used with the kits PK-MB20-600-50, PK-MB20-400-100, and PK-MB20-410-100.