Products

Luminescent Calcium Indicators

Aequorin is a protein that contains coelenterazine as a luminescent compound and can be used for intracellular calcium ion detection. When three Ca2+ ions bind to the aequorin complex consisting of the 22 KD apoaequorin protein (APO), molecular oxygen and the luminophore coelenterazine, the latter is oxidized to coelenteramide with a concomitant release of CO2 and blue light. Since the intensity of bioluminescence emitted by aequorin upon calcium binding correlates with the Ca2+ concentration, a sensitive measurement of Ca2+ concentrations with a broad detection range (~0.1 µM to >100 µM) is possible. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence.

PromoKine offers native coelenterazine as well as several synthetic coelenterazine analogs for reconstituting aequorin in cells that have been transfected with apoaequorin-cDNA. The available coelenterazine derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex and aequorins containing the cp, f or h forms of coelenterazine generate a 10–20 times stronger luminescence than that of the aequorin formed from native coelenterazine (recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence intensity, with both a high quantum yield and a fast response time).

Product Name Description Size Catalog Number Information Prices
Coelenterazin 400a Coelenterazine; luminescent calcium indicator & luciferase substrate (also known as DeepBlue C, a trademark of Perkin Elmer) 50 µg PK-CA707-10125 click to select country
Coelenterazine cp Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10112 click to select country
Coelenterazine f Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10114 click to select country
Coelenterazin fcp Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10117 click to select country
Coelenterazin h Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10111 click to select country
Coelenterazin hcp Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10113 click to select country
Coelenterazine i Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10121 click to select country
Coelenterazine ip Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10116 click to select country
Coelenterazin, 2-methyl Coelenterazine derivative; luminescent calcium indicator & luciferase substrate. Has been reported to be a superior antioxidant for cells against reactive oxygen species (ROS) such as singlet oxygen and superoxide anion. 50 µg PK-CA707-10122 click to select country
Coelenterazin n Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10115 click to select country
Coelenterazine (native) Coelenterazine; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10110 click to select country
Coelenterazine Sampler Kit Coelenterazine and derivatives thereof 9 x 25 µg PK-CA707-10123 click to select country