Products

Intracellular Calcium Indicators

Calcium ions are important second messengers in cells. They are involved in muscle contraction, nerve signal transmission, immune cell activation, hormone secretion, etc.
Fluorescent calcium indicators selectively bind to calcium ions and show a spectral response upon binding Ca2+, i.e., fluorescence intensity or wavelength are altered. This shift of fluorescence wavelength and change of intensity upon calcium chelation can be monitored using fluorescence microscopy.

PromoKine offers a wide range of calcium indicators for sensitively detecting changes in the intracellular Ca2+ concentration, including the fluorescent Fura-2, Fluo-3, Rhod-2, and Indo-1 indicators (as cell-impermeable salts or cell-permeable AM esters) as well as the non-fluorescent calcium indicator Bapta.

Moreover, we are also providing a range of coelenterazine derivatives which are effective luminescent calcium indicators.

Indicator Name Mwt.1 Detection Mode2Kd(nM)3

Bis-Fura-24

779

Ex: 340/380 nm

370

Fluo-3

770

Em: 525 nm

390

Fura-2

642

Ex: 340/380 nm

145

Indo-1

650

Em: 405/485 nm

230

Mag-Fura-2

435

Ex: 340/380 nm

250.000

Rhod-2

755

Em: 578 nm

1000

 
1 Molecular weights for free acid form of indicators are listed.
2 Indicators for which a pair of wavelength are listed can be used for ratiometric calcium measurements.
3
Calcium dissociation constant measured at 22°C in pH 7.2 buffer.
4 Bis-Fura-2 has similar calcium response as fura-2 but with a 75% larger extinction coefficient.

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
5,5'-difluoro-Bapta (tetrapotassium salt) Fluorescent calcium indicator 100 mg PK-CA707-50006 click to select country
5,5'-difluoro-Bapta AM Fluorescent calcium indicator (acetoxymethyl ester) 25 mg PK-CA707-50005 click to select country
Bis-Fura-2, hexapotassium salt Fluorescent calcium indicator 1 mg PK-CA707-50045 click to select country
Fluo-3 (pentaammonium salt) Fluorescent calcium indicator; Ex/Em: 490-506/526 1 mg PK-CA707-50010 click to select country
Fluo-3 (pentapotassium salt) Fluorescent calcium indicator; Ex/Em: 490-506/526 1 mg PK-CA707-50011 click to select country
Fluo-3 (pentasodium salt) Fluorescent calcium indicator; Ex/Em: 490-506/526 1 mg PK-CA707-50012 click to select country
Fluo-3 AM Fluorescent calcium indicator (acetoxymethyl ester) 1 mg PK-CA707-50014 click to select country
Fluo-3 AM (special packaging) Fluorescent calcium indicator (acetoxymethyl ester) 20 x 50 µg PK-CA707-50016 click to select country
Fluo-3 AM (special packaging) Fluorescent calcium indicator (acetoxymethyl ester) 10 x 100 µg PK-CA707-50013 click to select country
Fluo-3 AM Solution (1 mM in anhydrous DMSO) Fluorescent calcium indicator (acetoxymethyl ester; 1 mM in anhydrous DMSO) 1 ml PK-CA707-50015 click to select country
Fura-2 (pentaammonium salt) Fluorescent calcium indicator; Ex/Em: 340-380/510 1 mg PK-CA707-50030 click to select country
Fura-2 (pentapotassium salt) Fluorescent calcium indicator; Ex/Em: 340-380/510 1 mg PK-CA707-50031 click to select country
Fura-2 (pentasodium salt) Fluorescent calcium indicator; Ex/Em: 340-380/510 1 mg PK-CA707-50032 click to select country
Fura-2 AM Fluorescent calcium indicator (acetoxymethyl ester) 1 mg PK-CA707-50034 click to select country
Fura-2 AM (special packaging) Fluorescent calcium indicator (acetoxymethyl ester) 20 x 50 µg PK-CA707-50033-1 click to select country
Fura-2 AM (special packaging) Fluorescent calcium indicator (acetoxymethyl ester) 10 x 100 µg PK-CA707-50033 click to select country
Fura-2 AM Solution (1 mM in anhydrous DMSO) Fluorescent calcium indicator (acetoxymethyl ester; 1 mM in anhydrous DMSO) 1 ml PK-CA707-50029 click to select country
Furaptra, AM ester Fluorescent calcium and magnesium indicator (also known as Mag-Fura-2) 1 mg PK-CA707-50038 click to select country
Furaptra, AM ester (special packaging) Fluorescent calcium and magnesium indicator (also known as Mag-Fura-2) 10 x 100 µg PK-CA707-50037 click to select country
Furaptra, AM ester (special packaging) Fluorescent calcium and magnesium indicator (also known as Mag-Fura-2) 20 x 50 µg PK-CA707-50039 click to select country
Furaptra, tetrapotassium salt Fluorescent calcium and magnesium indicator (also known as Mag-Fura-2) 1 mg PK-CA707-50035 click to select country
Furaptra, tetrasodium salt Fluorescent calcium and magnesium indicator (also known as Mag-Fura-2) 1 mg PK-CA707-50036 click to select country
Indo-1 (pentaammonium salt) Fluorescent calcium indicator; Ex/Em: 330/405-485 1 mg PK-CA707-50040 click to select country
Indo-1 (pentapotassium salt) Fluorescent calcium indicator; Ex/Em: 330/405-485 1 mg PK-CA707-50041 click to select country
Indo-1 (pentasodium salt) Fluorescent calcium indicator; Ex/Em: 330/405-485 1 mg PK-CA707-50042 click to select country
Indo-1 AM Fluorescent calcium indicator (acetoxymethyl ester) 1 mg PK-CA707-50044 click to select country
Indo-1 AM (special packaging) Fluorescent calcium indicator (acetoxymethyl ester) 20 x 50 µg PK-CA707-50043-1 click to select country
Indo-1 AM (special packaging) Fluorescent calcium indicator (acetoxymethyl ester) 10 x 100 µg PK-CA707-50043 click to select country
Rhod-2 (triammonium salt) Fluorescent calcium indicator; Ex/Em: 533-556/~578 1 mg PK-CA707-50020 click to select country
Rhod-2 (tripotassium salt) Fluorescent calcium indicator; Ex/Em: 533-556/~578 1 mg PK-CA707-50021 click to select country
Rhod-2 (trisodium salt) Fluorescent calcium indicator; Ex/Em: 533-556/~578 1 mg PK-CA707-50022 click to select country
Rhod-2 AM Fluorescent calcium indicator (acetoxymethyl ester) 1 mg PK-CA707-50024 click to select country
Rhod-2 AM (special packaging) Fluorescent calcium indicator (acetoxymethyl ester) 10 x 100 µg PK-CA707-50023 click to select country