- Cytotoxicity assays
- Chemosensitivity testing
- Toxicity and drug screening
- Non-radioactive method
- Highly sensitive
- Easy and fast procedure
- Detection and quantification using spectrophotometer, fluorometer, or luminometer
- Compatible to various multiwell formats and suited for high-throughput assays
Colorimetric and fluorometric LDH Cytotoxicity Kits
Based on the activity of lactate dehydrogenase (LDH), our colorimetric and fluorometric LDH Cytotoxicity Kits provide a fast, sensitive, and precise alternative to the radioactive [3H]-thymidine and [51Cr] release assays.
LDH is a stable cytoplasmic enzyme present in all cells and is rapidly released into the surrounding medium when cells are damaged. In this assay, LDH activity is determined by a coupled enzymatic reaction. LDH catalyses the conversion of lactate to pyruvate, triggering an enzymatic reaction that produces either a water-soluble red formazan dye (colorimetric LDH Cytotoxicity Kit II) or an intense fluorescent product (fluorometric LDH Cytotoxicity Kit I). The increase in the amount of formazan or fluorescence produced in culture medium correlates directly to the increase in the number of lysed cells and can be analyzed spectrophotometrically or fluorometrically. The kits can be used for many different in vitro cell systems to quantify cytotoxicity and cytolysis in fast and convenient assays.
Cell Proliferation/Cytotoxicity Kit II
PromoKine’s Cell Proliferation/Cytotoxicity Kit II (fluorometric) provides an easy, rapid and quantitative method to measure cell proliferation as well as cytotoxicity. The assay is based on a nuclear dye that specifically binds to nucleic acid in the cell nucleus and emits green fluorescence. The generated fluorescent intensity is directly proportional to the cell number (viable or dead cells), which can be quantified by measuring fluorescence at Ex/Em = 480/538 nm using a fluorometer or fluorescence plate reader. This assay kit provides a just add-and-read, non-radioactive, and high-throughput method that is more sensitive than MTT, XTT, or MTS-based assays, and can detect a wide linear range of 25-60,000 cells.
Bioluminescent AK Cytotoxicity Kit
The bioluminometric AK Cytotoxicity Kit is based on the measurement of adenylate kinase (AK), a ubiquitous protein present in all cells that is rapidly released into the culture medium upon damage to the plasma membrane. The simple one-step procedure involves two chemical reactions. First, the adenylate kinase released from the damaged cells catalyses the conversion of ADP to ATP. The second reaction utilizes luciferase to catalyze a reaction between the ATP, oxygen, and luciferin producing light. The light can be measured using a luminometer or beta counter. This highly sensitive assay allows rapid quantitation of plasma membrane damage for evaluation of cell death or cytotoxicity events, and it is well suited for automation and high throughput.