Products

Fluorometric Cell Viability Kits

Fluorometric Cell Viability Kit I (Resazurin)

The resazurin-based PromoKine Cell Viability Kit I provides a simple and rapid measurement of cell viability. Moreover, this single-step, homogenous cell proliferation assay is sensitive, safe, and cost-effective. It only requires as few as 80 cells for reproducible results. The redox dye Resazurin (also known as Alamar Blue) detects cell viability by converting from a non-fluorescent, blue dye to the highly red fluorescent dye resorufin in response to chemical reduction of the growth medium caused by cell growth. The fluorescent or colorimetric signal generated from the assay is proportional to the number of living cells in the sample. The kit reagents are nontoxic to cells and allow kinetic monitoring. While fluorometric resorufin quantitation is more sensitive, colorimetric measurement is also possible.

Fluorometric Cell Viability Kit II (Calcein)

The PromoKine Fluorometric Cell Viability Kit II is intented for the reliable fluorometric determination of viable cell numbers. It utilizes the cell membrane-permeable, non-fluorescent dye Calcein-AM that is hydrolyzed by esterases to the cell membrane-impermeable green-fluorescent Calcein. Since esterases are only active in viable cells, the amount of Calcein-AM hydrolyzed by cellular esterases is directly proportional to the number of viable cells in culture.

Figure: Assay mechanism of Calcein-AM conversion by intracellular esterases. (Click on image for enlarging it).

 

 

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
Fluorometric Cell Viability Kit I (Resazurin; 2500 assays) Fluorometric cell viability and cytotoxicity detection 25 ml PK-CA707-30025-1 click to select country
Fluorometric Cell Viability Kit I (Resazurin; 500 assays) Fluorometric cell viability and cytotoxicity detection 5 ml PK-CA707-30025-0 click to select country
Fluorometric Cell Viability Kit I (Resazurin;10000 assays) Fluorometric cell viability and cytotoxicity detection 100 ml PK-CA707-30025-2 click to select country
Fluorometric Cell Viability Kit II (Calcein) Fluorometric cell viability and cytotoxicity detection 1000 assays PK-CA707-30026 click to select country
Resazurin, sodium salt Can be used for measuring cell proliferation and mitochondrial metabolic activity 10 mg PK-CA707-10054 click to select country


Try our viability assays!

Smallest size of the Resazurin Kit  (500 assays) is available with a unique test discount!