The Colorimetric Cell Viability Kits I and II allow for easy and reliable colorimetric determination of viable cell numbers with excellent sensitivity and linearity. The kits utilize the tetrazolium salts WST-8 (Kit I) and WST-1 (Kit II) that are reduced to water-soluble, orange formazan dyes by dehydrogenases present in viable cells. The absorbance of the formazan dye is proportional to the number of metabolically active cells and can be measured directly from a 96-well plate without additional processing.
The PromoKine Colorimetric Cell Viability Kit III has been developed for determining the number of viable cells in culture. It is based on the reduction of the tetrazolium salt XTT into a colored formazan compound by mitochondrial enzymes. Since these enzymes are inactivated shortly after cell death, this is a reliable method for the detection of viable cells. The absorbance of the formazan product can be measured directly from a 96-well plate without additional processing.
In particular, the use of XTT greatly simplifies the procedure of measuring proliferation, and is, therefore, and excellent solution for the quantitating of cells and their viability without using radioactive isotopes. The PromoKine Cell Viability Kit III (XTT) was developed to assay cell proliferation in response to different growth factors, cytokines and nutrient components. In addition, it is suitable for assaying cytotoxicity of materials such as TNF or other growth inhibitors. XTT can be used as a non-radioactive substitute for cytotoxic tests based on the release of 51Cr from cells with no less sensitivity.
Determination of live cell numbers is often used to assess the rate of cell proliferation and cell cytotoxicity caused by drugs and cytotoxic agents. The PromoKine Cell Viability Kit IV (MTT) provides a simple method for determining live cell numbers using a standard colorimetric plate reader. Among all non-radioactive viability assays, the MTT assay developed by Mossman is one of the most versatile and popular assays. MTT is a tetrazolium salt that is converted into a purple formazan product after reduction by mitochondrial enzymes that are only present in metabolically active live cells, but not in dead cells. The amount of formazan product generated is proportional to the number of living cells in the sample. The formazan product can be solubilized and then photometrically quantified at 570 nm.
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* WST-8 Cell Viability Asaay is currently not available in Japan, China and Korea.
