Products

Bioluminescent Cell Viability Kits

Bioluminescent Cell Viability Kit I (ATP)

The Promokine Bioluminescent Cell Viability Kit I employs bioluminescence to measure the ATP level as a marker for cell viability in mammalian cells. It utilizes the enzyme luciferase to catalyze the ATP-dependent, light-producing oxidation of luciferin. This light formation can be measured using a luminometer or beta counter.

The assay can be performed directly in culture plates requiring no harvest, washing, or sample preparation and can be fully automated for high throughput (10 seconds/sample). It is highly sensitive, detecting 10-100 mammalian cells/well, and it also offers new applications besides cell viability testing, e.g., detecting low level bacterial contamination in blood, milk, urine, soil, and sludge.

 

Bioluminescent Cell Viability Kit II (ADP/ATP)

Changes in the ADP/ATP ratio are a good indicator for cell viability and can also be used to differentiate between apoptotic and necrotic cell death. High ATP and low ADP levels are usually found in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are characteristic for apoptotic cells. In necrotic cells, the ADP levels are even higher, and ATP can be barely detected.

The Bioluminescent Cell Viability Kit II utilizes bioluminescence to measure the ADP/ATP ratio providing an innovative system for fast, simultaneous screening of cell proliferation, apoptosis, necrosis, and growth arrest in mammalian cells. The ATP level is determined using luciferin-luciferase reaction described in the Bioluminescent Cell Viability Kit I. The ADP level is measured by its conversion to ATP, which is subsequently detected using the same luciferase-catalyzed reaction. The assay can be fully automated for high throughput and is highly sensitive, detecting as few as 100 mammalian cells/well. It offers highly consistent results with excellent correlation to other apoptosis markers (e.g., TUNEL-based or caspase assays).  

 

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
Bioluminescent Cell Viability Kit I (ATP) This kit utilizes bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells 200 assays PK-CA577-K254-200 click to select country
Bioluminescent Cell Viability Kit I (ATP) This kit utilizes bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells 1000 assays PK-CA577-K254-1000 click to select country
Bioluminescent Cell Viability Kit II (ADP/ATP) Assay for detecting ADP/ATP ratios for a rapid screening of apoptosis/necrosis/growth arrest/cell proliferation simultaneously in mammalian cells 200 assays PK-CA577-K255 click to select country