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Fluorometric & Colorimetric ATP & ADP Quantitation Kits

ATP that is formed exclusively in the mitochondria is the major energy currency of living systems and virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. A variety of genetic diseases can affect ATP formation in the mitochondria. There are a number of commercially available ATP assays which detects femtomoles or less of ATP by measuring luminescence (PromoKine Kit PK-CA577-254-200, for example) but these kits require specialized luminescence instrumentation and utilize luciferase which can be difficult to maintain in active form. PromoKine's new Colorimetric & Fluorometric ATP Quantitation Kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (λmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect as low as 50 picomol (1 µM) of ATP in various samples.

 

ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast, although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. PromoKine’s new Fluorometric & Colorimetric ADP Quantitation Kit provides an convenient colorimetric and fluorometric means to measure the ADP level.  In this assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified colorimetrically (λmax = 570 nm) or fluorometrically (Ex/Em 535/587 nm). The assay is simple, sensitive, stable and high-throughput adaptable and can detect as low as 1 µM ADP in biological samples.

Product Name Description Size Catalog Number Information Prices
Fluorometric & Colorimetric ADP Quantitation Kit For convenient measurement of ADP level in cells 100 assays PK-CA577-K355 click to select country
Fluorometric & Colorimetric ATP Quantitation Kit For convenient measurement of ATP level in cells 100 assays PK-CA577-K354 click to select country