Fluorometric & Colorimetric GST Assay Kits
SOD Assay Kit
Fluorometric & Colorimetric Nitric Oxide Kits
HAT Activity Kit
DNA Damage Detection Kit
NAD/NADH & NADP/NADPH Quantitation

Fluorometric & Colorimetric GST Assay Kits

Glutathione (GSH) is the major intracellular low-molecular-weight thiol and plays a critical role in cellular defense against oxidative stress in tissues and cells. Glutathione S-transferase (GST) is an essential enzyme involved in detoxification of xenobiotics mediating conjugation of glutathione with those compounds.

The Fluorometric and Colorimetric GST Assays can detect GST activity in crude cell lysate or purified protein fractions and the kits also suited for quantitation of GST-tagged fusion proteins. They are based upon the GST-catalyzed reaction between GSH and the GST substrate CDNB (Colorimetric Kit) or MCB (Fluorometric Kit).

CDNB has the broadest range of isozyme detectability (e.g. α-, µ-, phi-, and other GST isoforms). Under certain conditions, the interaction between glutathione and CDNB is totally dependent on the presence of active GST. Detection limit is approximately 10 ng active GST/assay.

The free form of MCB is almost non-fluorescent, whereas the dye fluoresces blue (Ex/Em = 380/461 nm) when reacting with glutathione. GST catalyzes the MCB-glutathione reaction, and the fluorescence levels are proportional to the amount of GST present in the reaction. Therefore, the GST level in samples can be easily detected using a fluorometer or a 96-well fluorometric plate reader.

Fluorometric & Colorimetric Nitric Oxide Kits

Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune responses, and apoptosis. PromoKine's Fluorometric and Colorimetric Nitric Oxide Kits provide an accurate and convenient tool for total nitrate/nitrite concentration determination in a simple two-step process that can be used as a quantitative measure of NO production. The first step is the conversion of nitrate to nitrite utilizing nitrate reductase. The second step involves the addition of the fluorometric reagent DAN followed by NaOH, which converts nitrite into a fluorescent compound (Fluorometric Nitric Oxide Kits) - or the addition of the Griess Reagent which converts nitrite into a deep purple azo compound (Colorimetric Nitric Oxide Kits). Measurement of the fluorescent compound reaction product or azo chromophore accurately determines the total nitric oxide production. The Fluorometric Assay Kit has been validated in culture media, plasma, and tissue homogenates. 

HAT Activity Kit

Histone acetyltransferases (HATs) have been implicated in playing a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. The colorimetric HAT Activity Kit offers a convenient, non-radioactive system for a rapid and sensitive, spectrophotometric detection of HAT activity in mammalian samples. The detection can be continuous and is therefore suitable for kinetic studies.

DNA Damage Detection Kit

Different physiological and environmental factors such as chemical induced damage and cell aging are causing DNA lesions. AP sites (apurinic/apyrimidinic sites) are one of the major types of DNA lesions formed during the process of base excision and repair of oxidized, deaminated, or alkylated bases. It has been estimated that about 2x10⁵ base lesions are generated per cell per day. For this reason, the level of AP sites in cells can be a good indicator of DNA lesions and repairs.
The DNA Damage Detection Kit utilizes the ARP (Aldehyde Reactive Probe) reagent that reacts specifically with the aldehyde group in the open ring form of the AP sites. After treating DNA containing AP sites with ARP reagents, AP sites are tagged with biotin residues, which can be quantified using an avidin-biotin assay followed by a colorimetric detection. The kit provides the necessary reagents for convenient determination of abasic sites in purified DNA sample in 96-well plate format.

NAD/NADH & NADP/NADPH Quantitation Kits

Note: Oxidative Stress is also topic of a training course of our PromoCell Academy! >More Information