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ROS Indicators

Reactive oxygen species (ROS) play a critical role in cellular physiopathology and are implicated for example in cell proliferation, signaling pathways, thyroid hormonosynthesis, as well as oxidative defense mechanisms (e.g. killing of bacteria). In several diseases such as cancer or neurodegenerative and cardiovascular disorders, the redox balance is disturbed and excessive amounts of ROS can damage cellular proteins, lipids and DNA.
Thus, having a sensitive method for detection of intracellular ROS is very important for detecting the disturbed redox balance in pathological cells as well as cells that have been exposed to different genotoxic agents (chemicals, irradiation, oxidative stress etc.).

The cell-permeant 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) is a chemically reduced form of fluorescein. It is commonly used as an indicator for reactive oxygen species (ROS) in cells, e.g. to detect the generation of reactive oxygen intermediates in neutrophils and macrophages. It passively diffuses into cells and is retained in the intracellular level after cleavage by intracellular esterases. Upon oxidation by ROS, the non-fluorescent H2DCFDA is converted to the highly fluorescent 2',7'-dichlorofluorescein (DCF).

Product Name Description Size Catalog Number Information Prices
H2DCFDA (H2-DCF, DCF) 2',7'-Dichlorodihydrofluorescein diacetate; Indicator of Reactive Oxygen Species (ROS) 100 mg PK-CA707-10058 click to select country