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Fluorometric & Colorimetric Lactate Assay Kits

Lactate plays an important roles in many biological processes. Abnormally high levels of lactate have been related to various disease states such as diabetes and lactic acidosis. L(+)-Lactate is the major lactate stereoisomer formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate.

In the colorimetric L-Lactate Assay Kit I, lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (Abs.max = 450 nm). The kit detects L(+)-Lactate in biological samples such as serum or plasma, cell culture and fermentation media at a level of 0.02 mM – 10 mM. There is no need for pretreatment or purification of samples.

In the fluorometric/colorimetric L-Lactate Assay Kit II, lactate specifically reacts with an enzyme mix to generate a product, which interacts with a lactate probe to produce a signal detectable as a color (570 nm) or as fluorescence (Ex/Em = 535/587 nm). The kit provides a convenient means for detecting L(+)-Lactate in various biological samples, e.g. in circulating blood, cells, culture media, fermentation media, etc. at concentrations between 0.001 -10 mM. There is no need of pre-treatment or purification of samples.

PromoKine’s colorimetric D-Lactate Assay Kit I provides a fast, easy way to accurately measure D-Lactate in a variety of biological samples: D-Lactate is specifically oxidized by D-Lactate dehydrogenase and generates a proportional color signal (Abs.max = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cell culture and fermentation media. The useful concentration range in samples is 0.01 mM – 10 mM D-Lactate.

The fluorometric D-Lactate Assay Kit II offers simplicity, high sensitivity, and can be adapted to high-throughput research. The assay enzymatically oxidizes D-Lactate generating a fluorescent signal (Ex/Em = 535/587 nm), which is directly proportional to the amount of D-Lactate present in the sample. Highly sensitive, this assay can detect D-Lactate in samples containing as low as 0.1 µM.

Product Name Description Size Catalog Number Information Prices
D-Lactate Assay Kit I Colorimetric detection of D-Lactate in various biological samples 100 Assays PK-CA577-K667 click to select country
D-Lactate Assay Kit II Fluorometric detection of D-Lactate in various biological samples 100 assays PK-CA577-K668 click to select country
L-Lactate Assay Kit I Colorimetric detection of L-Lactate in various biological samples 100 Assays PK-CA577-K627 click to select country
L-Lactate Assay Kit II Fluorometric and colorimetric detection of L-Lactate in various biological samples 100 Assays PK-CA577-K607 click to select country