- Colorimetric measurement of histone acetyltransferase activity in mammalian samples
- Highly sensitive and reproducible detection/measurement
- Ready-to-use and convenient assays
- Suited for high-throughput applications
HAT Activity Assay Kits
Histone acetyltransferases (HATs) have been implicated in playing a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. The colorimetric HAT Activity Kit offers a convenient, non-radioactive system for a rapid and sensitive, spectrophotometric detection of HAT activity in mammalian samples. The detection can be continuous and is therefore suitable for kinetic studies.
PromoKine’s fluorometric HAT Activity Assay Kit II utilizes Acetyl-CoA and H3 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer to generate a product that is detected fluorometrically at a high sensitivity.