HAT Activity Assay Kits

Histone acetyltransferases (HATs) have been implicated in playing a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. The colorimetric HAT Activity Kit offers a convenient, non-radioactive system for a rapid and sensitive, spectrophotometric detection of HAT activity in mammalian samples. The detection can be continuous and is therefore suitable for kinetic studies.

PromoKine’s fluorometric HAT Activity Assay Kit II utilizes Acetyl-CoA and H3 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer to generate a product that is detected fluorometrically at a high sensitivity.

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
HAT Activity Assay Kit I Detects HAT activity by colorimetric method in mammalian samples 100 assays PK-CA577-K332 click to select country
HAT Activity Assay Kit II Highly sensitive fluorometric detection of HAT activity in mammalian samples 100 Assays PK-CA577-K334 click to select country
HAT protein (P/CAF, active) Recombinant, active histone acetyltransferase (HAT) 100 µg PK-RP577-1137-100 click to select country