Products

Mitochondria & ER Stains

Mitochondria generate ATP as an energy source in cell metabolism,and because they have electron transport systems, they can be stained with various redox dyes. Mitochondrial dyes are typically small organic dyes bearing a positive charge. The dyes rapidly cross the plasma membrane and stain mitochondrial membranes. For some of the dyes, staining intensity is dependent on  mitochondrial membrane potential (MOMP), making these dyes useful for assessing mitochondrial function in live cells (e.g.the 488 nm-excitable JC-1 dye used for analysing apoptosis). The fluorescence intensity of Rhodamine 123 which readily passes through cell membranes and accumulates in mitochondria, reflects the amount of ATP generated there. Other mitochondrial dyes, such as NAO, are relatively insensitive to mitochondrial membrane potential, but are useful for staining mitochondria in fixed cells or for measuring mitochondrial mass.

MitoGreen is a green fluorescent mitochondrial dye with properties similar to those of MitoTracker Green FM. It is spectrally similar to FITC, making it optimally excitable by the 488 nm argon laser line. The dye is nonfluorescent until it partitions into the mitochondrial membrane. The staining dye can be used to stain mitochondria in both live cells and fixed cells. Live cells stained with the dye may be fixed with formaldehyde.

MitoRed is a far-red fluorescent mitochondrial dye (Ex/Em=622/648 nm). The staining is dependent on mitochondrial membrane potential. This dye can be used to stain mitochondria in live cells, and is not fixable.

Synthesis and sorting of cellular proteins and lipids occurs in the endoplasmic reticulum (ER) and Golgi apparatus. PromoKine offers a short-chain carbocyanine dye for ER labelling [DiOC6(3)] and fluorescent NBD C6 ceramide for selective staining of the Golgi apparatus in live and fixed cells.

Product Name Description Size Catalog Number Information Prices
4-Di-1-ASP (DASPMI) 4-(4-(Dimethylamino)-styryl)-N-methylpyridinium iodide; stains mitochondria in live cells 500 mg PK-CA707-70006 click to select country
DASPEI 2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide; for staining of mitochondria in live cells 100 mg PK-CA707-70018 click to select country
Dihydrorhodamine 123 Reduced form of Rhodamine 123; mitochondria stain 10 mg PK-CA707-10055 click to select country
Dihydrorhodamine 123, dihydrochloride salt Reduced form of Rhodamine 123; mitochondria stain 10 mg PK-CA707-10056 click to select country
DiOC6(3) 3,3’-Dihexyloxacarbocyanine, iodid; membrane potential sensitive dye; also suited for ER staining 100 mg PK-CA707-70009 click to select country
DODC (DiOC2(5)) Nucleic acid binding, lipophilic carbocyanine dye (high purity) 50 mg PK-CA707-40040 click to select country
JC-1 chloride Stains mitochondria in living cells in a membrane potential-dependent function 5 mg PK-CA707-70011 click to select country
JC-1 iodide Stains mitochondria in living cells in a membrane potential-dependent function 5 mg PK-CA707-70014 click to select country
MitoGreen Green-fluorescent mitochondrial dye 20 x 50 µg PK-CA707-70054 click to select country
MitoRed Red-fluorescent mitochondrial dye 20 x 50 µg PK-CA707-70055 click to select country
NBD C6-ceramide 6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosine 1 mg PK-CA707-60030 click to select country
Nonyl Acridine Orange (or NAO) Mitochondria stain; not dependent on mitochondrial membrane potential 50 mg PK-CA707-70012 click to select country
Rhodamine 123 Green-fluorescent mitochondria stain 50 mg PK-CA707-70010 click to select country
Tetrabromorhodamine 123, bromide Green-fluorescent mitochondria stain 5 mg PK-CA707-70013 click to select country
TMRE Tetramethylrhodamine ethyl ester, perchlorate; membrane potential sensitive dye 25 mg PK-CA707-70016 click to select country
TMRM Tetramethylrhodamine methyl ester, perchlorate; membrane potential sensitive dye 25 mg PK-CA707-70017 click to select country