Applications
- Quantitative determination of cAMP/cGMP levels
- Cell signalling and cell growth/differentiation studies
- β-Secretase activity detection
- Alzheimer's disease drug development
Features
- Fast and simple assays
- Highly sensitive
Cell Signaling Assays
cAMP and cGMP Immunoassay Kits
Adenosine 3,5-cyclic monophosphate (cyclic AMP, cAMP) is one of the most important second messengers involved as a modulator in physiological processes regulating neuronal, glandular, and cardiovascular processes as well as immune mechanisms, the nervous system, cell growth and differentiation. A number of hormones (e.g. ACTH, TSH, LH) are known to activate cAMP through the action of the enzyme adenylate cyclase which converts ATP to cAMP.
Guanosine 3,5-cyclic monophosphate (cyclic GMP, cGMP) has been shown to be present at levels typically 10-100 fold lower than cAMP in most tissues and is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones (e.g. acetylcholine, insulin, and oxytocin) and chemicals (e.g. serotonin and histamine) cause an increase in cGMP levels.
beta-Secretase Fluorometric Assay Kit
β-Secretase (BACE) is a membrane-bound aspartyl protease that cleaves the amyloid precursor protein and is consequently an excellent target for anti-amyloid therapy in the treatment of Alzheimer's disease. Finding inhibitors of β-secretase is one of the major goals of Alzheimer's disease drug development. PromoKine's β-Secretase Fluorometric Assay Kit provides a convenient, non-radioactive system for detecting β-Secretase activity in biological samples. The kit provides active β-Secretase as positive control, β-Secretase inhibitor as negative control, optimized peptide substrate (conjugated to two reporter molecules), and buffers for convenient measurement of β-Secretase activity in mammalian samples. β-Secretase inhibitors are also available separately.