Products

Cell Invasion Assay Kits

Cell invasion is a highly integrated, multi-step process by which cells migrate from one location to another penetrating a physiological barrier composed of extracellular matrix proteins in response to chemoattractants and/or inhibiting compounds.
Cell motility is observed in unicellular organisms and is essential for the development and maintenance of multicellular organisms. Cells often migrate in response to specific external stimuli, including chemical and mechanical signals.
As cell migration/invasion plays an important role in various cellular processes such as angiogenesis, embryonic development, and the immune response, alterations in this process can lead to a number of pathologies including vascular disease, tumor
formation and metastasis, atherosclerosis, arthritis and cognitive impairment.

PromoKine’s Cell Invasion Assay Kits are easy to use, sensitive and adaptable to highthroughput systems.The Cell Invasion Assay Kits also utilize a Boyden chamber coated with a layer of Basement Membrane Extract (BME, growth factor-reduced and endotoxin-free), laminin, Collagen I or Collagen IV. The cells first traverse the matrix in a combination of protein degradation and cellular locomotion and then migrate through the semi-permeable membrane in the Boyden chamber in response to different stimuli.

Cell invasion can be analyzed directly by reading fluorescence (Ex/Em = 530 nm/590nm) in a plate reader.  

Selection Guide Cell Migration & Cell Invasion Assays

Principle of PromoKine‘s Cell Invasion Assays. 1) Plate well with insert; cell suspension in top chamber, media with or without chemoattractants in bottom chamber. 2) Invading cells migrate through a porous polycarbonate membrane coated with a barrier membrane and cling to the outer side of the top chamber. Non-invasive cells stay in the top chamber. 3) Invasive cells are dissociated from the membrane into the media of the bottom chamber, fluorescently stained and quantified using a fluorescence plate reader.
Product Name Description Size Catalog Number Information Prices
Cell Invasion Assay Kit (BME, 8 µm, 24-well) Highly sensitive and quantitative fluorometric assay to measure cell invasion in response to stimuli. 24 assays PK-CA577-K913 click to select country
Cell Invasion Assay Kit (BME, 8 µm, 96-well) Highly sensitive and quantitative fluorometric assay to measure cell invasion in response to stimuli. 100 assays PK-CA577-K912 click to select country
Cell Invasion Assay Kit (laminin, 8 µm, 24-well) Highly sensitive and quantitative fluorometric assay to measure cell invasion in response to stimuli. 24 assays PK-CA577-K915 click to select country
Cell Invasion Assay Kit (laminin, 8 µm, 96-well) Highly sensitive and quantitative fluorometric assay to measure cell invasion in response to stimuli. 100 assays PK-CA577-K914 click to select country
Cell Invasion Assay Kit (collagen I, 8 µm, 24-well) Highly sensitive and quantitative fluorometric assay to measure cell invasion in response to stimuli. 24 assays PK-CA577-K917 click to select country
Cell Invasion Assay Kit (collagen I, 8 µm, 96-well) Highly sensitive and quantitative fluorometric assay to measure cell invasion in response to stimuli. 100 assays PK-CA577-K916 click to select country
Cell Invasion Assay Kit (collagen IV, 8 µm, 24-well) Highly sensitive and quantitative fluorometric assay to measure cell invasion in response to stimuli. 24 assays PK-CA577-K919 click to select country
Cell Invasion Assay Kit (collagen IV, 8 µm, 96-well) Highly sensitive and quantitative fluorometric assay to measure cell invasion in response to stimuli. 100 assays PK-CA577-K918 click to select country