- Isolation of total cellular protein
- Isolation of membrane protein
- Separation of cellular fractions and enrichment of distinct protein fractions
- Convenient and efficient cell fractionation and protein extraction
- Easy protocols
- Proteins and cell fractions can be used for many downstream applications
Cell Fractionation Kits
PromoKine also offers kits for convenient fractionation of cellular proteins into distinct subcellular groups or for the isolation of intact, functional mitochondria.
Our diverse Cell Fractionation and Separation Kits contain unique formulations of buffers and reagents for convenient preparation of highly enriched cellular fractions (mitochondria, cytosol, nuclear) from mammalian cells.
The Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear and cytoplasmic fractions of mammalian cells and tissues with little or no cross-contaminations, maintaining the nuclear and cytoplasmic compartments separate and intact.
The Mitochondria/Cytosol Fractionation Kit allows isolation of a highly enriched mitochondrial fraction from the cytosol of mammalian cells in a simple and staightforward procedure without ultracentrifugation and toxic chemicals.
The Cytosol/Particulate Separation Kit provides an easy and convenient system for a rapid separation of cytosol and particulate fractions. The separated fractions are compatible with downstream applications, such as assaying cellular distributions of small molecules or proteins and examining other signal transduction pathways.
The Complete Cell Fractionation Kit is designed for serial sample preparation of four distinct protein fractions including cytosol, particulate, cytoskeleton, and nuclear fractions from one sample.
The prepared fractions are suitable for many downstream assays, such as two-dimensional gel electrophoresis, protein immunoblotting, gel-shift assays, enzyme activity assays, reporter assays, and more.
PromoKine's Mitochondria Isolation Kit (for tissue & cultured cells) offers two options for the isolation of intact mitochondria. The first option is utilizing a reagent-based method allowing parallel processing of multiple samples. The second option uses traditional dounce homogenization, which provides better mitochondrial yield.