Cell Fractionation & Protein Extraction Kits
Efficient protein isolation from different cell compartments
Applications
- Cellular protein extraction from mammalian cells
- Integral membrane protein extraction
- Preparation of highly enriched cellular fractions (mitochondria, cytosol, nuclear) from mammalian cells
Benefits
- Convenient and efficient cell fractionation and protein extraction
- Easy protocols
- Proteins and cell fractions can be used for many downstream applications
Mammalian Whole Cell Protein Extraction Kit
The Mammalian Whole Cell Protein Extraction Kit has been optimized for complete extraction of all cellular proteins. It contains an optimized lysis buffer and a protease inhibitor cocktail as well as other reagents for convenient extraction of mammalian proteins from tissues and cells under non-denaturing conditions. The entire procedure takes less than 20 minutes, and the proteins can be used for many downstream applications such as gel electrophoresis, Western blotting, etc.
Membrane Protein Extraction Kit
The Membrane Protein Extraction Kit provides an easy and complete protocol for isolating integral membrane proteins efficiently from cultured mammalian cells. The kit has been designed to not only extract the total cellular membrane proteins, but also to specifically purify the plasma membrane proteins. The procedure offers consistent yield and high purity (> 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.
Cell Fractionation Kits
PromoKine also offers kits for convenient fractionation of cellular proteins into distinct subcellular groups.
Our diverse Cell Fractionation and Separation Kits contain unique formulations of buffers and reagents for convenient preparation of highly enriched cellular fractions (mitochondria, cytosol, nuclear) from mammalian cells.
The Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear and cytoplasmic fractions of mammalian cells and tissues with little or no cross-contaminations, maintaining the nuclear and cytoplasmic compartments separate and intact.
The Mitochondria/Cytosol Fractionation Kit allows isolation of a highly enriched mitochondrial fraction from the cytosol of mammalian cells in a simple and staightforward procedure without ultracentrifugation and toxic chemicals.
The Cytosol/Particulate Separation Kit provides an easy and convenient system for a rapid separation of cytosol and particulate fractions. The separated fractions are compatible with downstream applications, such as assaying cellular distributions of small molecules or proteins and examining other signal transduction pathways.
The Complete Cell Fractionation Kit is designed for serial sample preparation of four distinct protein fractions including cytosol, particulate, cytoskeleton, and nuclear fractions from one sample.
The prepared fractions are suitable for many downstream assays, such as two-dimensional gel electrophoresis, protein immunoblotting, gel-shift assays, enzyme activity assays, reporter assays, and more.
- PromoKine’s RTU Protein Ladder I separated on different SDS polyacrylamide gels.
Protein Gel Stain & Protein Markers
GelQuick Protein Staining/Destaining Kit
Traditional staining and destaining methods for SDS-PAGE protein gels are known to take several hours and also leave a high blue background which reduces the sensitivity of band detection.
PromoKine’s GelQuick Gel Stain/Destaining Kit provides a fast and sensitive method for SDS-PAGE gel staining and destaining. The procedure only takes 5 minutes for gel staining and 15 minutes for destaining. After a total of 20 minutes visualization of as little as 5 ng of protein on a waterclear background can be seen.
The kit contains sufficient solutions to stain and destain up to 40 pieces of protein mini gels.
Prestained RTU Protein Ladders
PromoKine’s Prestained RTU Protein Ladders are very cost-effective and supplied ready-to-use for direct loading onto gels. They can be used for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, estimation of protein sizes, and verification of Western transfer efficiency onto membranes (PVDF, nylon, or nitrocellulose).
They show excellent stability with sharp bands, and allow easy identification as bands of different colors are used. Each Prestained RTU Protein Ladder is sufficient for 100-200 mini gels.
The Prestained RTU Protein Ladder I contains 9 pink-stained protein markers covering the range from 10 to 175 kDa and three blue-stained reference bands at 10, 40 and 90 kDa.
The Prestained RTU Protein Ladder II is a three-color protein standard covering a broad range (10-245 kDa) with ten blue-stained protein bands and two reference bands at 25 kDa (green) and 75 kDa (red).
Order Information
Note: Protein Purification is also topic of various training courses of our PromoCell Academy! >More Information