Products

Luciferase Reporter Assay Kits

Firefly and Renilla luciferases are widely used as reporter genes for studying gene regulation and function, and for pharmaceutical screening. PromoKine's ready-to-use Luciferase Reporter Assay Kits provide complete systems for rapid, convenient, and quantitative detection of Firefly and/or Renilla luciferase luciferase activity in cultured eukaryotic cells, e.g., cells transfected with a plasmid carrying the Firefly and/or Renilla luciferase reporter genes. They are optimized to provide excellent sensitivity as well as consistent light output when working with multiple samples.
Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. Consequently, this sensitive reporter is ideally suited for studying gene regulation and function.
Renilla luciferase is often used in conjunction with Firefly luciferase as a normalizing transfection control or for multiplex transcriptional reporter assays. Here, our improved Luciferase Reporter Assay Kit III (Firefly & Renilla Single-tube Assay) provides optimal convenience and highest sensitivity.
Both, Firefly luciferase and Renilla luciferase show maximum light output only when substrates and co-factors are present in large quantities. When assayed under these conditions, light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules.  The light emission from the reaction can be recorded using a luminometer.

The Luciferase Reporter HTS Kits have  been specifically designed for economic, high-throughput applications undeer standard laboratory conditions using high-density microplates. It is a homogenous glow-type assay with a signal half-life of 3-5 hours allowing to test several thousand samples per hour with high reproducibility and without prior sample processing.

In addition to the Firefly luciferase substrate D-Luciferin, we also provide a wide range of coelenterazine substrates for the Renilla luciferase catalysed reaction (see order information).

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
Luciferase Reporter Assay Kit I (Firefly) Ready-to-use kit providing a complete system for a rapid, convenient and quantitative detection of Firefly luciferase activity in eukaryotic cells. 1000 assays PK-CA707-30003-2 click to select country
Luciferase Reporter Assay Kit I (Firefly) Ready-to-use kit providing a complete system for a rapid, convenient and quantitative detection of Firefly luciferase activity in eukaryotic cells. 150 assays PK-CA707-30003-1 click to select country
Luciferase Reporter Assay Kit II (Renilla) Ready-to-use kit providing a complete system for a rapid, convenient and quantitative detection of Renilla luciferase activity in eukaryotic cells. 1000 assays PK-CA707-30004-2 click to select country
Luciferase Reporter Assay Kit II (Renilla) Ready-to-use kit providing a complete system for a rapid, convenient and quantitative detection of Renilla luciferase activity in eukaryotic cells. 150 assays PK-CA707-30004-1 click to select country
Luciferase Reporter Assay Kit III (Firefly & Renilla Single-tube Assay) Ready-to-use kit providing a complete system for a rapid, convenient and quantitative detection of Firefly and Renilla luciferase activity in eukaryotic cells 1000 assays PK-CA707-30081-2 click to select country
Luciferase Reporter Assay Kit III (Firefly & Renilla Single-tube Assay) Ready-to-use kit providing a complete system for a rapid, convenient and quantitative detection of Firefly and Renilla luciferase activity in eukaryotic cells 150 assays PK-CA707-30081-1 click to select country
Luciferase Reporter HTS Kit (Firefly) Ready-to-use kit providing a complete system for high-throughput screening of Firefly luciferase activity in eukaryotic cells. 12 ml PK-CA707-30028-1 click to select country
Luciferase Reporter HTS Kit (Firefly) Ready-to-use kit providing a complete system for high-throughput screening of Firefly luciferase activity in eukaryotic cells. 4 ml PK-CA707-30028-0 click to select country
Coelenterazin 400a Coelenterazine; luminescent calcium indicator & luciferase substrate (also known as DeepBlue C, a trademark of Perkin Elmer) 50 µg PK-CA707-10125 click to select country
Coelenterazin fcp Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10117 click to select country
Coelenterazin h Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10111 click to select country
Coelenterazin hcp Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10113 click to select country
Coelenterazin n Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10115 click to select country
Coelenterazin, 2-methyl Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10122 click to select country
Coelenterazine (native) Coelenterazine; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10110 click to select country
Coelenterazine cp Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10112 click to select country
Coelenterazine f Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10114 click to select country
Coelenterazine i Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10121 click to select country
Coelenterazine ip Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 µg PK-CA707-10116 click to select country
Coelenterazine Sampler Kit Coelenterazine and derivatives thereof 9 x 25 µg PK-CA707-10123 click to select country
D-Luciferin, Free Acid Natural substrate of luciferase from firefly, that is primarily used in reporter assays and ATP assays. 1 g PK-CA707-10100-2 click to select country
D-Luciferin, Free Acid Natural substrate of luciferase from firefly, that is primarily used in reporter assays and ATP assays. 50 mg PK-CA707-10100-1 click to select country
D-Luciferin, Free Acid Natural substrate of luciferase from firefly, that is primarily used in reporter assays and ATP assays. 10 mg PK-CA707-10100 click to select country
D-Luciferin, Potassium Salt Natural substrate of luciferase from firefly, that is primarily used in reporter assays and ATP assays. 1 g PK-CA707-10101-2 click to select country
D-Luciferin, Potassium Salt Natural substrate of luciferase from firefly, that is primarily used in reporter assays and ATP assays. 50 mg PK-CA707-10101-1 click to select country
D-Luciferin, Potassium Salt Natural substrate of luciferase from firefly, that is primarily used in reporter assays and ATP assays. 10 mg PK-CA707-10101 click to select country
D-Luciferin, Sodium Salt Natural substrate of luciferase from firefly, that is primarily used in reporter assays and ATP assays. 1 g PK-CA707-10102-2 click to select country
D-Luciferin, Sodium Salt Natural substrate of luciferase from firefly, that is primarily used in reporter assays and ATP assays. 50 mg PK-CA707-10102-1 click to select country
D-Luciferin, Sodium Salt Natural substrate of luciferase from firefly, that is primarily used in reporter assays and ATP assays. 10 mg PK-CA707-10102 click to select country
DMNPE-caged D-Luciferin D-luciferin, 1-(4,5-dimethoxy-2-nitrophenyl)ethyl ester 5 mg PK-CA707-10103 click to select country
5X Passive Lysis Buffer To be used with PromoKine's Renilla Luciferase Assay Kit (catalog no. PK-CA707-30004) or Firefly & Renilla Luciferase Single-tube Assay Kit (catalog no. PK-CA707-30081). 30 ml PK-CA707-99912 click to select country
5X Firefly Lysis Buffer To be used with PromoKine's Firefly Luciferase Assay Kit (catalog no. PK-CA707-30003-1/-2) or Firefly & Renilla Luciferase Single-tube Assay Kit (catalog no. PK-CA707-30081). 15 ml PK-CA707-99923 click to select country
Firefly Luciferase Stabilizer, 2.5X To be added to Passive Lysis Buffer if purified recombinant firefly luciferase enzyme is used (PromoKine's Firefly Luciferase Assay & Renilla/Firefly Luciferase Assay). 20 ml PK-CA707-30083 click to select country