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3D Cell Culture - Cell Harvesting & Viability Assay Kits

Isolating intact cells in 3D cell culture from the extracellular matrix proteins comprising the 3D matrices in order to use them for cell passaging or biochemical and molecular analysis can be very difficult. While proteases (such as trypsin and accutase) are commonly used to degrade these matrices, this is not recommended in some cases where cells are sensitive to protease digestion as these proteases may modify cell surface and/or cellular proteins and signaling, thus altering physiological assessments. Further, some protease-based dissociation methods do not completely dissolve the matrices.

PromoKine’s 3D Cell Culture Harvesting Kit provides an optimized and standardized saline-based solution for a simplified non-enzymatic isolation of cells and spheroids from matrices (especially for PromoKine’s 3D Cell Culture Kits: PK-CA577-K517, -K518, -K519) with high viability rates for subsequent biochemical, protein and cell-based analysis. This kit provides reagents for extracting cells and/or cell aggregates from 3D cell cultures for biochemical analysis, as well as reagents for the preparation of lysates for immunoblotting.

Quantification of viable cell numbers is an indispensable tool in in vitro screening in many biological and pharmacological studies. Calcein-AM is a non-fluorescent compound that easily penetrates intact and live cells and does fluoresce brightly green once the AM group is cleaved off by intracellular esterases. It has been widely used to assess cell viability and proliferation in cell biology research. However, with the use of 3D matrices, some protease-based dissociation methods do not completely dissolve the matrices and cell aggregates, which can distort results of quantitative in vitro assays such as viability assessment. PromoKine’s 3D Cell Culture Viability Assay Kit provides an easy-to-use, high-throughput and sensitive method for screening, characterizing and studying cell viability, and cytotoxicity in three-dimensional cell cultures. The standardized fluorometric method reliably quantifies viable cells detecting as low as 50 viable cells per well. The measured fluorescence intensity at Ex/Em = 485/530 nm is directly proportional to the number of viable cells. The complete kit comes with an optimized and gentle non-enzymatic dissociation solution for the recovery of viable and dead cells from spheroids in matrices and scaffolds.     

Product Name Description Size Catalog Number Information Prices
3D Cell Culture Harvesting Kit Provides an optimized and standardized saline-based solution for the isolation of cells and spheroids from matrices with high viability rate for subsequent biochemical, protein and cell-based analysis. 100 samples PK-CA577-K982 click to select country
3D Cell Culture Viability Assay Kit Provides a standardized fluorometric method for sensitive quantification of viable cells in 3D cell culture. 100 assays PK-CA577-K948 click to select country