Applications

Screening for apoptotic cells
Kinase detection and activity screening

Features

Non-radioactive method
Easy, accurate and fast procedure
Compatible with standard lab equipment

JNK Activity Screening Kit

The JNK Activity Screening Kit is used to screen JNK activity quickly - also for large numbers of samples. The assay utilizes an N-terminal c-Jun fusion protein bound to glutathion sepharose beads to selectively isolate JNK from cell lysate. After a washing step to remove nonspecifically bound proteins, the kinase reaction is carried out in the presence of cold ATP. c-Jun phosphorylation is then determined by Western blot analysis using a phospho-c-Jun specific antibody.

Product Name	Description	Size	Catalog Number
c-Jun (1-79)-GSTFusion Protein		100 µg	PK-CA577-7001
GSK-3a-GST Fusion Protein	Recombinant protein fusion of GSK-3-alpha ?residues surrounding GSK-3-alpha ?(Ser21) and glutathione S-transferase (GST)	100 µg	PK-CA577-7003
GSK-3b, human, recombinant	N-terminal His-tagged human full-length recombinant GSK-3-beta	100 µg	PK-CA577-7004
JNK Activated Cell Lysate	Anisomycin treated Jurkat cell lysate (positive control for JNK assay)	1 mg	PK-CA577-7032
JNK Activity Screening Kit	Complete kit for quick screening of JNK activity	40 assays	PK-CA577-K430
JNK Negative Control Cell Lysate	Untreated Jurkat Cell Lysate (negative control for JNK activity assays)	1 mg	PK-CA577-7031
L-JNK Peptide Inhibitor I (1 mM)	Ready-to-use and cell permeable JNK peptide inhibitor	50 µl	PK-CA577-7011
L-JNKI1 Negative Control Peptide (1 mM)	Ready-to-use and cell permeable JNKI1 Negative Control Peptide	50 µl	PK-CA577-7021