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Kinase Detection
Applications
- Screening for apoptotic cells
- Kinase detection and activity screening
Benefits
- Non-radioactive method
- Easy, accurate and fast procedure
- Compatible with standard lab equipment
The activation of protein kinases, such as JNK and Akt, plays an important role in apoptosis and other physiological and pathological processes. PromoKine provides a novel nonradioactive system for convenient measurement of JNK and Akt activities.
JNK Activity Screening Kit
The JNK Activity Screening Kit is used to screen JNK activity quickly - also for large numbers of samples. The assay utilizes an N-terminal c-Jun fusion protein bound to glutathion sepharose beads to selectively isolate JNK from cell lysate. After a washing step to remove nonspecifically bound proteins, the kinase reaction is carried out in the presence of cold ATP. c-Jun phosphorylation is then determined by Western blot analysis using a phospho-c-Jun specific antibody.
JNK Activity Immunoassay Kit
The JNK Activity Immunoassay Kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. Activity of the JNK is then detected in a kinase reaction using recombinant c-Jun as substrate. c-Jun phosphorylation is determined by Western blotting using a phospho-c-Jun specific antibody. The kit specifically detects JNK activity; other kinase activities would not be detected.
Akt Activity Immunoassay Kit
The Akt Activity Immunoassay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Akt- specific activity is then detected in a kinase reaction using recombinant GSK-3α; as substrate. Phosphorylation levels of GSK-3α are determined by Western blot analysis using a phospho-GSK-3α specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.
Individual Kinase Reagents
A wide range of active kinases (including JNK and Akt kinases) and kinase antibodies as well as kinase substrates, activators, inhibitors and control cell lysates are also available.
Kinase Antibodies (coming soon)
Control Cell Lysates (on request)