- Screening for apoptotic cells
- Glutathione detection
- Quantitation of glutathione reductase activity
- Non radioactive, highly sensitive method
- Easy and fast procedure
- Compatible with common spectrophotometers/ fluorometers and plate readers
Glutathione Detection Kits
Fluorometric Glutathione Detection Kit
Diminished glutathione (GSH) levels occur at the early stages of apoptosis. The PromoKine Fluorometric Glutathione Detection Kit provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes. The assay utilizes a dye (monochlorobimane, MCB) that has a high affinity for glutathione. The unbound dye is almost nonfluorescent; whereas the dye fluoresces blue (excitation = 380 nm; emission = 461 nm) when bound to reduced or oxidized glutathione. The reaction is catalyzed by glutathione S-transferase (GST). Thus, the decrease in glutathione level in apoptotic cells can be easily detected using a fluorometer or a 96-well fluorometric plate reader.
Colorimetric Glutathione Detection Kit
The Colorimetric Glutathione Detection Kit provides a convenient, colorimetric method for analyzing either total glutathione or only the reduced form of glutathione using a microtiter plate reader. The assay utilizes the glutathione recycling system with DTNB and glutathione reductase: GSH is regenerated from GSSG by glutathione reductase, and will again react with DTNB to produce yellow 2-nitro-5-thiobenzoic acid that can be determined spectrophotometrically at 412 nm. This recycling reaction dramatically improves the sensitivity of total glutathione detection - the kit can quantify total glutathione from 1-100 ng/well. For detecting lower glutathione concentrations, such as in blood samples, increasing reaction time will significantly improve the signal. The kit can also specifically detect the reduced form of glutathione (GSH) by omitting the glutathione reductase from the reaction mixture.
Colorimetric Glutathione Reductase Activity Kit
PromoKine’s Colorimetric Glutathione Reductase Activity Kit has been designed for measuring glutathione reductase (GR) activity in biological samples. It is highly sensitive and well suited for high-throughput. The assay is based on the GR-catalysed reduction of GSSG to GSH, which reacts with 5,5′-Dithiobis(2-nitrobenzoic acid) (DTNB) to generate the yellow TNB2-. The kit can detect 0.1 – 40 mU/ml GR in various samples.
References, Questions & Answers
For Glutathione S-Transferase (GST) Assays Kits click here.