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Granzyme B Activity Assay & Inhibitor Screening Kits

Granzyme B also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.

PromoKine’s Granzyme B Activity Assay Kit utilizes a peptide substrate containing the Granzyme B recognition sequence along with a fluorescent label ‘AFC’. Granzyme B catalyzes the cleavage of this substrate and releases the AFC molecule, which can be detected fluorometrically (Ex/Em = 380/500 nm). This Assay Kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.

In PromoKine’s Granzyme B Inhibitor Screening Kit, we also have utilized a peptide substrate containing the Granzyme B recognition sequence along with a fluorescent label ‘AFC’. Granzyme B catalyzes the cleavage of this substrate and releases the AFC molecule, which can be detected fluorometrically. In the presence of a potent Granzyme B inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive and reliable test suitable for high-throughput screening of Granzyme B inhibitors or characterizing/studying Granzyme B inhibitors. Inhibitor control, Ac-IEPD-CHO is included to compare the efficacy of test inhibitors.

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
Fluorometric Granzyme B Activity Assay Kit For measurement of Granzyme B activity in various biological samples 100 Assays PK-CA577-K168 click to select country
Fluorometric Granzyme B Inhibitor Screening Kit For screening and characterizing Granzyme B inhibitors 100 Assays PK-CA577-K169 click to select country
Granzyme B Substrate Z-AAD-R110 Provided in the ready-to-use form 5 mg PK-CA707-10211 click to select country
Granzyme B Inhibitor Ac-IEPD-CHO Provided in the ready-to-use form 1 mg PK-CA577-1119 click to select country
Granzyme B Inhibitor Z-AAD-CH2Cl (10 mM) Granzyme B Inhibitor Z-AAD-CMK (chloromethylketone); 10 mM 20 µl PK-CA577-1128-20C click to select country