Products

Cathepsin Activity Assay & Inhibitor Screening Kits

The Cathepsin Activity Assay Kits are based on fluorometric or colorimetric (Cathepsin G assay) detection and utilize the preferred substrate sequence for each cathepsin, labeled with AFC (amino-4-trifluoromethylcoumarin), MCA (7-amino-4-methyl coumarin) or pNA (p-nitroaniline). Cell lysates and other samples that contain cathepsins cleave the synthetic substrate to release free AFC, MCA or pNA that can be easily quantified using a fluorometer/photometer or a fluorescence plate reader/ELISA reader. The cathepsin assays are easy to perform and can be adapted to 96-well plate assays. Assay conditons have been optimized to obtain the maximal activity.

The fluorometric Cathepsin Inhibitor Screening Kits include active Cathepsin enzymes and a synthetic peptide substrate for the respective Cathepsin which is coupled to AFC or MCA. The cathepsin cleaves the synthetic substrate and releases the fluorescent group, which can then easily be measured using a fluorometer or fluorescence plate reader. These are simple, straightforward assays that can be adapted to 96-well plates and high-throughput screening. The relative efficacy of test inhibitors which reduce/abolish cleavage of the substrate are compared to positive control inhibitors included in the kit.

 

For Cathepsin L ELISA Kit please click here.

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
Cathepsin B Activity Assay Kit For measuring cathepsin B activity by fluorometer or fluorescence plate reader 100 assays PK-CA577-K140 click to select country
Cathepsin B Inhibitor Screening Kit Fluorometric kit to for fast screening of potential cathepsin B (CTSB) inhibitors. 100 assays PK-CA577-K147 click to select country
Cathepsin D Activity Assay Kit For measuring cathepsin D activity by fluorometer or fluorescence plate reader 100 assays PK-CA577-K143 click to select country
Cathepsin D Inhibitor Screening Kit Allows fast screening of potential cathepsin D inhibitors 100 assays PK-CA577-K148 click to select country
Cathepsin E Activity Assay Kit For measuring cathepsin E activity by fluorometer or fluorescence plate reader 100 assays PK-CA577-K165 click to select country
Cathepsin E Inhibitor Screening Kit Fluorometric kit to for fast screening of potential cathepsin E inhibitors. 100 assays PK-CA577-K166 click to select country
Cathepsin G Activity Assay Kit For measuring cathepsin G activity by photometer or ELISA plate reader 100 assays PK-CA577-K146 click to select country
Cathepsin G Inhibitor Screening Kit Colorimetric kit to for fast screening of potential cathepsin G inhibitors. 100 assays PK-CA577-K162 click to select country
Cathepsin H Activity Assay Kit For measuring cathepsin H activity by fluorometer or fluorescence plate reader 100 assays PK-CA577-K145 click to select country
Cathepsin H Inhibitor Screening Kit Fluorometric kit to for fast screening of potential cathepsin H inhibitors. 100 assays PK-CA577-K164 click to select country
Cathepsin K Activity Assay Kit For measuring cathepsin K activity by fluorometer or fluorescence plate reader 100 assays PK-CA577-K141 click to select country
Cathepsin K Inhibitor Screening Kit Fluorometric kit to for fast screening of potential cathepsin K inhibitors. 100 assays PK-CA577-K150 click to select country
Cathepsin L Activity Assay Kit For measuring cathepsin L activity by fluorometer or fluorescence plate reader 100 assays PK-CA577-K142 click to select country
Cathepsin L Inhibitor Screening Kit Fluorometric kit to for fast screening of potential cathepsin L inhibitors. 100 assays PK-CA577-K161 click to select country
Cathepsin S Activity Assay Kit For measuring cathepsin S activity by fluorometer or fluorescence plate reader 100 assays PK-CA577-K144 click to select country
Cathepsin S Inhibitor Screening Kit Fluorometric kit to for fast screening of potential cathepsin S inhibitors. 100 assays PK-CA577-K149 click to select country
Cathepsin G Inhibitor A potent, selective, reversible, competitive, non-peptide inhibitor of cathepsin G. 1 mg PK-CA577-1982-1 click to select country
Cathepsin G Substrate (colorimetric) An excellent colorimetric substrate for cathepsin G. 5 mg PK-CA577-2206-5 click to select country
Cathepsin G Substrate (colorimetric) An excellent colorimetric substrate for cathepsin G. 25 mg PK-CA577-2206-25 click to select country
Cathepsin Substrate Z-FR-AMC (Z-FR-MCA) Substrate for Serine Proteases; provided in the ready-to-use form 25 mg PK-CA707-10201 click to select country
Cathepsin B & L Substrate Z-FR-R110 Provided in the ready-to-use form 5 mg PK-CA707-10209 click to select country
Cathepsin B&L Inhibitor Z-Phe-Phe-FMK (10 mM) Provided in the ready-to-use form 20 µl PK-CA577-1126-20C click to select country
Procathepsin Activation Buffer For generating active Cathepsins from inactive Procathepsins and testing activity or inhibition of Cathepsins. 1 ml PK-CA577-1030-1 click to select country
Procathepsin B, human, recombinant Recombinant Human Procathepsin B 10 µg PK-RP577-7579-10 click to select country
Procathepsin B, human, recombinant Recombinant Human Procathepsin B 50 µg PK-RP577-7579-50 click to select country
Cathepsin B, human, recombinant Recombinant Human Cathepsin B (active) 5 µg PK-RP577-7580-5 click to select country
Cathepsin B, human, recombinant Recombinant Human Cathepsin B (active) 50 µg PK-RP577-7580-50 click to select country
Cathepsin B, human (native) native Active Human Cathepsin B 5 µg PK-RP577-1021 click to select country
Cathepsin D, human, recombinant RecombinantHuman Cathepsin D (active) 10 µg PK-RP577-7409-10 click to select country
Cathepsin D, human (native) native Active Human Cathepsin D 5 µg PK-RP577-1022 click to select country
Cathepsin G, human (native) Active Cathepsin G derived from human neutrophils 100 µg PK-RP577-4713-100 click to select country
Cathepsin H, human (native) native Active Human Cathepsin H 5 µg PK-RP577-1023 click to select country
Procathepsin K, human, recombinant Human recombinant Procathepsin K 10 µg PK-RP577-1026-10 click to select country
Procathepsin K, human, recombinant Human recombinant Procathepsin K 50 µg PK-RP577-1026-50 click to select country
Procathepsin K, mouse recombinant Mouse recombinant Procathepsin K 10 µg PK-RP577-1027-10 click to select country
Procathepsin K, mouse, recombinant Mouse recombinant Procathepsin K 50 µg PK-RP577-1027-50 click to select country
Procathepsin K, rat, recombinant Recombinant Rat Procathepsin K 10 µg PK-RP577-1029-10 click to select country
Procathepsin K, rat, recombinant Recombinant Rat Procathepsin K 50 µg PK-RP577-1029-50 click to select country
Cathepsin K, human, recombinant Recombinant Human Cathepsin K (active) 5 µg PK-RP577-7600-5 click to select country
Cathepsin K, mouse, recombinant recombinant Recombinant Mouse Cathepsin K (active) 5 µg PK-RP577-7597-5 click to select country
Cathepsin K, rat, recombinant recombinant Recombinant Rat Cathepsin K (active) 5 µg PK-RP577-7598-5 click to select country
Procathepsin L, human, recombinant Recombinant Human Procathepsin L 5 µg PK-RP577-7581-5 click to select country
Procathepsin L, human, recombinant Recombinant Human Procathepsin L 100 µg PK-RP577-7581-100 click to select country
Cathepsin L, human, recombinant Active Human Cathepsin L 5 µg PK-RP577-1135-5 click to select country
Cathepsin L, human, recombinant Active Human Cathepsin L 100 µg PK-RP577-1135-100 click to select country
Procathepsin S, human, recombinant Recombinant Human Procathepsin S 10 µg PK-RP577-7525-10 click to select country
Procathepsin S, human, recombinant Recombinant Human Procathepsin S 50 µg PK-RP577-7525-50 click to select country
Cathepsin S, human, recombinant Recombinant Human Cathepsin S (active) 10 µg PK-RP577-7526-10 click to select country
Cathepsin S, human, recombinant Recombinant Human Cathepsin S (active) 50 µg PK-RP577-7526-50 click to select country